The Brassica napus receptor‐like protein RLM2 is encoded by a second allele of the LepR3/Rlm2 blackleg resistance locus. Issue 7 (3rd February 2015)

• Record Type: Journal Article
• Authors: Larkan, Nicholas J.; Ma, Lisong; Borhan, Mohammad Hossein
• Is Part Of: Plant biotechnology journal. Volume 13:Issue 7(2015:Sep.)
• Page Start: 983
• Page End: 992
• Publication Date: 2015-02-03
• Subjects: Plant biotechnology -- Periodicals; Plant genetic engineering -- Periodicals; 630.272
• DOI: 10.1111/pbi.12341
• Languages: English
• ISSNs: 1467-7644

Abstract

<abstract abstract-type="main" id="pbi12341-abs-0001"> <title>Summary</title> <p>Leucine‐rich repeat receptor‐like proteins (LRR‐RLPs) are highly adaptable parts of the signalling apparatus for extracellular detection of plant pathogens. Resistance to blackleg disease of <italic>Brassica</italic> spp. caused by <italic>Leptosphaeria maculans</italic> is largely governed by host race‐specific <italic>R</italic>‐genes, including the LRR‐RLP gene <italic>LepR3</italic>. The blackleg resistance gene <italic>Rlm2</italic> was previously mapped to the same genetic interval as <italic>LepR3</italic>. In this study, the <italic>LepR3</italic> locus of the <italic>Rlm2</italic><italic>Brassica napus</italic> line 'Glacier DH24287' was cloned, and <italic>B. napus</italic> transformants were analysed for recovery of the <italic>Rlm2</italic> phenotype. Multiple <italic>B. napus</italic>, <italic> B. rapa</italic> and <italic>B. juncea</italic> lines were assessed for sequence variation at the locus. <italic>Rlm2</italic> was found to be an allelic variant of the <italic>LepR3</italic> LRR‐RLP locus, conveying race‐specific resistance to <italic>L. maculans</italic> isolates harbouring <italic>AvrLm2</italic>. Several defence‐related LRR‐RLPs have previously been shown to associate with the RLK SOBIR1 to facilitate defence signalling. Bimolecular fluorescence complementation (BiFC) and co‐immunoprecipitation of RLM2‐SOBIR1 studies revealed that RLM2 interacts with SOBIR1 of <italic>Arabidopsis thaliana</italic> when co‐expressed in <italic>Nicotiana benthamiana</italic>. The interaction of RLM2 with <italic>At</italic>SOBIR1 is suggestive of a conserved defence signalling pathway between <italic>B. napus</italic> and its close relative <italic>A. thaliana</italic>.</p> </abstract>