Effectiveness of Alpha-toxin Fab Monoclonal Antibody Therapy in Limiting the Pathology of Staphylococcus aureus Keratitis. (August 2015)

• Record Type: Journal Article
• Authors: Caballero, Armando R.; Foletti, Davide L.; Bierdeman, Michael A.; Tang, Aihua; Arana, Angela M.; Hasa-Moreno, Adela; Sangalang, Emma Ruth B.; O'Callaghan, Richard J.
• Is Part Of: Ocular immunology and inflammation. Volume 23:Number 4(2015)
• Page Start: 297
• Page End: 303
• Publication Date: 2015-08
• Subjects: Eye -- Diseases -- Immunological aspects -- Periodicals; Eye -- Inflammation -- Periodicals; 617.7
• Languages: English
• ISSNs: 0927-3948

Abstract

<abstract> <title>Abstract</title> <p> <italic>Purpose</italic>: To investigate the effectiveness of a high-affinity human monoclonal antibody Fab fragment to <italic>Staphylococcus aureus</italic> alpha-toxin (LTM14 Fab) as therapy for <italic>S. aureus</italic> keratitis.</p> <p> <italic>Methods</italic>: A single topical drop of the LTM14 Fab antibody to alpha-toxin alone, or in 0.006% benzalkonium chloride (BAK), was applied every 30 min to <italic>S. aureus</italic>-infected rabbit corneas from 9 to 14 hours post-infection. Erosions and pathology were measured at 15 h post-infection.</p> <p> <italic>Results</italic>: LTM14 Fab with BAK limited corneal erosions better than LTM14 Fab alone (<italic>p</italic> = 0.036), and both limited erosions compared to untreated eyes (<italic>p</italic> ≤ 0.0001). Overall pathology was similar in all groups (<italic>p</italic> ≥ 0.070), but iritis and chemosis were reduced by treatment (<italic>p</italic> ≤ 0.036).</p> <p> <italic>Conclusions</italic>: The high-affinity human monoclonal Fab fragment antibody (LTM14 Fab) to <italic>S. aureus</italic> alpha-toxin was effective in reducing corneal damage during <italic>S. aureus</italic> keratitis.</p> </abstract>