The minor ecdysteroids from Ajuga turkestanica. Issue 5 (8th May 2015)

• Record Type: Journal Article
• Authors: Guibout, Louis; Mamadalieva, Nilufar; Balducci, Christine; Girault, Jean‐Pierre; Lafont, René
• Is Part Of: Phytochemical analysis. Volume 26:Issue 5(2015:Sep.)
• Page Start: 293
• Page End: 300
• Publication Date: 2015-05-08
• Subjects: Plants -- Analysis -- Periodicals; Plants -- chemistry -- Periodicals; 572.2
• DOI: 10.1002/pca.2563
• Languages: English
• ISSNs: 0958-0344

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="pca2563-sec-0001" sec-type="section"> <title>Introduction</title> <p> <italic>Ajuga turkestanica</italic> is a plant used in traditional medicine for its high ecdysteroid content, including the presence of the particularly active turkesterone, which possess efficient anabolic activity.</p> </sec> <sec id="pca2563-sec-0002" sec-type="section"> <title>Objectives</title> <p>To isolate and identify minor ecdysteroids present in a semi‐purified plant fraction containing ca. 70% turkesterone.</p> </sec> <sec id="pca2563-sec-0003" sec-type="section"> <title>Material and Methods</title> <p>Multi‐step preparative HPLC (combining RP‐ and NP‐HPLC systems) was used to purify the different components present in the turkesterone fraction. Isolated compounds were identified by high‐resolution mass spectrometry and 2D‐NMR.</p> </sec> <sec id="pca2563-sec-0004" sec-type="section"> <title>Results</title> <p>Fourteen ecdysteroids (including turkesterone and 20‐hydroxyecdysone) were isolated. Seven of these, all bearing an 11α‐hydroxy group, were previously unreported.</p> </sec> <sec id="pca2563-sec-0005" sec-type="section"> <title>Conclusion</title> <p> <italic>Ajuga turkestanica</italic> ecdysteroids are characterised by the abundance of 11α‐hydroxylated compounds and by the simultaneous presence of 24C, 27C, 28C and 29C ecdysteroids. It is expected that even more ecdysteroids are to be found in this plant since the starting material for this study lacked the less polar ecdysteroids. The simultaneous presence of 20‐hydroxyecdysone and turkesterone (its 11α‐hydroxy analogue) as the two major ecdysteroids suggests that every ecdysteroid is probably present in both 11α‐hydroxy and 11‐deoxy forms. Copyright © 2015 John Wiley & Sons, Ltd.</p> </sec> </abstract>