Selective Antimicrobial and Antibiofilm Disrupting Properties of Functionalized Diamond Nanoparticles Against Escherichia coli and Staphylococcus aureus. (1st June 2015)

• Record Type: Journal Article
• Authors: Khanal, Manakamana; Raks, Viktoria; Issa, Rahaf; Chernyshenko, Volodymyr; Barras, Alexandre; Garcia Fernandez, Jose M.; Mikhalovska, Lyuba I.; Turcheniuk, Volodymyr; Zaitsev, Vladimir; Boukherroub, Rabah; Siriwardena, Aloysius; Cooper, Ian R.; Cragg, Peter J.; Szunerits, Sabine
• Is Part Of: Particle and particle systems characterization. Volume 32:Number 8(2015:Aug.)
• Page Start: 822
• Page End: 830
• Publication Date: 2015-06-01
• Subjects: Particles -- Periodicals; 620.43
• DOI: 10.1002/ppsc.201500027
• Languages: English
• ISSNs: 0934-0866

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Diamond nanoparticles (NDs) have demonstrated great promise as useful materials in a variety of biomedical settings. In this paper, the antimicrobial and antibiofilm activities of variously functionalized NDs against two common bacterial targets Gram‐negative bacterium <italic>Escherichia coli</italic> and Gram‐positive bacterium <italic>Staphylococcus aureus</italic> are compared. Hydroxylated (ND‐OH), aminated (ND‐NH<sub>2</sub>), carboxylated (ND‐COOH), mannose (ND‐Mannose), tri‐thiomannoside (ND‐Man<sub>3</sub>), or tri‐thiolactoside (ND‐Lac<sub>3</sub>)‐modified NDs are fabricated and evaluated in the present work. Of these, the mannose‐modified NDs are found to interfere most strongly with the survival of <italic>S. aureus</italic>, but not to influence the growth of <italic>E. coli</italic>. In contrast, particles featuring lactosyl units have the opposite effect on <italic>S. aureus</italic> growth. Sugar‐functionalized NPs reported to display antibacterial effects are rare. Only ND‐COOH particles are seen to have any effect on the growth profile of <italic>E. coli</italic>, but the effects are moderate. On the other hand, both ND‐NH<sub>2</sub> and ND‐COOH are found to inhibit <italic>E. coli</italic>‐induced biofilm formation at levels comparable to the known <italic>E. coli</italic> biofilm disruptor, ampicillin (albeit at concentrations of 100 μg mL<sup>−1</sup>). However, none of the modified particles examined here reveal any significant activity as disruptors of <italic>S. aureus</italic>‐induced biofilm formation even at the highest concentrations studied.</p> </abstract>