Histological variety of gastric carcinoma and Helicobacter pylori cagA and vacA polymorphism. Issue 9 (September 2015)

• Record Type: Journal Article
• Authors: Figura, Natale; Valassina, Marcello; Moretti, Elena; Vindigni, Carla; Collodel, Giulia; Iacoponi, Francesca; Giordano, Nicola; Roviello, Francesco; Marrelli, Daniele
• Is Part Of: European journal of gastroenterology & hepatology. Volume 27:Issue 9(2015:Sep.)
• Publication Date: 2015-09
• Subjects: Digestive organs -- Diseases -- Periodicals; Liver -- Diseases -- Periodicals; Digestive organs -- Diseases; Liver -- Diseases; Periodicals; 616.33
• DOI: 10.1097/MEG.0000000000000414
• Languages: English
• ISSNs: 0954-691X

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <sec> <title>Background</title> <p> <italic>Helico</italic> <italic>bacter pylori</italic> infection may increase the risk of both histotypes of gastric carcinoma (GC): intestinal (IGC) and diffuse (DGC). Polymorphism of the main <italic>H. pylori</italic> virulence factors, <italic>cagA</italic> and <italic>vacA</italic>, could have a different impact on the histological variety of GC.</p> </sec> <sec> <title>Methods</title> <p>Two hundred and twenty-six <italic>H. pylori</italic> colonies were examined from 28 GC patients: 15 with IGC and 13 with DGC. DNA was extracted from bacteria and a PCR was performed using primers specific for <italic>cagA</italic>, the 3′ <italic>cagA</italic> variable region and s and m regions of <italic>vacA</italic>.</p> </sec> <sec> <title>Results</title> <p>There were 214 <italic>cagA</italic>+ strains and 55.61% of them were isolated from IGC cases; there were 12 <italic>cagA</italic>− colonies and they were all isolated from DGC (<italic>P</italic><0.001). Most patients were infected by strains with more than one <italic>cagA</italic> structural type. No strains with a particular <italic>cagA</italic> type were found to be significantly more frequent in either histological variety of GC. A percentage of 43.90 of strains with <italic>vacA</italic> subtype s1/m1 were isolated from IGC, whereas 80.95% of <italic>vacA</italic> subtype s1/m2 strains were isolated from DGC cases (<italic>P</italic><0.001).</p> </sec> <sec> <title>Conclusion</title> <p>Polymorphism of the main virulence genes of <italic>H. pylori</italic> may play different roles in the pathogenesis of IGC and DGC.</p> </sec> </abstract>