Kiwifruit fermentation drives positive gut microbial and metabolic changes irrespective of initial microbiota composition. Issue 1 (July 2015)

• Record Type: Journal Article
• Authors: Blatchford, Paul; Stoklosinski, Halina; Walton, Gemma; Swann, Jonathan; Gibson, Glenn; Gearry, Richard; Ansell, Juliet
• Is Part Of: Bioactive carbohydrates and dietary fibre. Volume 6:Issue 1(2015)
• Page Start: 37
• Page End: 45
• Publication Date: 2015-07
• Subjects: Functional foods -- Periodicals; Fiber in human nutrition -- Periodicals; Carbohydrates -- Periodicals; Glycoproteins -- Periodicals; Functional Food -- Periodicals; Dietary Fiber -- Periodicals; Carbohydrates -- Periodicals; Carbohydrates; Fiber in human nutrition; Functional foods; Glycoproteins; Periodicals; 613.28
• DOI: 10.1016/j.bcdf.2015.07.001
• Languages: English
• ISSNs: 2212-6198

Abstract

<abstract abstract-type="author" id="ab0010"> <title id="sect0005">Abstract</title> <sec> <p id="sp0025">It is well established that individuals vary greatly in the composition of their core microbiota. Despite differing ecology, we show here that metabolic capacity converges under the pressure of kiwifruit substrates in a model gut system. The impact of pre-digested green and gold kiwifruit on the human colonic microbiota and their metabolic products was assessed using <italic>in vitro</italic>, pH-controlled, anaerobic batch culture fermenters. Phylogenetic analyses revealed that bacterial composition changed over time, irrespective of whether a substrate was added or not, indicating a natural adjustment period to the gut model environment. Adding kiwifruit substrate caused additional changes in terms of growth of specific bacterial groups, bacterial diversity and metabolite profiles. Relative abundance of <italic>Bacteroides</italic> spp. increased with both green and gold kiwifruit substrate while <italic>Bifidobacterium</italic> spp. increased only with green kiwifruit. NMR spectroscopy and GC demonstrated an increase in organic acids (primarily acetate, butyrate, and propionate) and a concomitant decrease in several amino acids and oligosaccharides following addition of green and gold kiwifruit substrate. The experiments demonstrated that despite markedly different baseline profiles in individual donor inoculum, kiwifruit components can induce substantive change in microbial ecology and metabolism which could have consequences for human health.</p> </sec> </abstract>