Transcriptional dynamics of immune, growth and stress related genes in skeletal muscle of the fine flounder (Paralichthys adpersus) during different nutritional statuses. Issue 1 (November 2015)

• Record Type: Journal Article
• Authors: Valenzuela, Cristián A.; Escobar, Daniela; Perez, Lorena; Zuloaga, Rodrigo; Estrada, Juan Manuel; Mercado, Luis; Valdés, Juan Antonio; Molina, Alfredo
• Is Part Of: Developmental and comparative immunology. Volume 53:Issue 1(2015)
• Page Start: 145
• Page End: 157
• Publication Date: 2015-11
• Subjects: Immunology -- Periodicals; Developmental immunology -- Periodicals; 616.079
• DOI: 10.1016/j.dci.2015.06.007
• Languages: English
• ISSNs: 0145-305X

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">The effects of stress on immune activity and growth in early vertebrates have not been studied in detail. The present study used fine flounder (<italic>Paralichthys adspersus</italic>) skeletal muscle as a model to evaluate molecules involved in the stress response, including the glucocorticoid receptors, <italic>foxo1/3</italic>, and the target genes of these. Additionally, immune markers (<italic>il-1β</italic> and <italic>tnfα</italic>) and effector molecules of atrophy (<italic>bnip3</italic>, <italic>caspase-3</italic>, and <italic>lc3</italic>) were assessed. These molecules were analyzed during periods of long-term fasting and refeeding. During fasting, gene expression related to the stress response and atrophy increased; whereas immune markers were down-regulated. During refeeding, atrophy- and stress-related gene expression significantly decreased. In contrast, immune markers were up-regulated. These results provide novel insight on the control of growth in the skeletal muscle of a non-mammalian species under a stressful condition, suggesting that growth, stress, and immune activity in muscle are closely related and coordinated by orchestrated transcriptional dynamics.</p> </sec> </abstract>