Discrimination between Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capricolum using PCR-RFLP and PCR. Issue 3 (September 2015)

• Record Type: Journal Article
• Authors: Cillara, Grazia; Manca, Maria Giovanna; Longheu, Carla; Tola, Sebastiana
• Is Part Of: Veterinary journal. Volume 205:Issue 3(2015)
• Page Start: 421
• Page End: 423
• Publication Date: 2015-09
• Subjects: Veterinary medicine -- Periodicals; 636
• DOI: 10.1016/j.tvjl.2015.05.013
• Languages: English
• ISSNs: 1090-0233

Abstract

<abstract abstract-type="author-highlights" id="ab0010"> <title id="st0010">Highlights</title> <sec> <p id="sp0010"> <list id="ulist0010"> <list-item id="u0010"> <label></label> <p id="p0010">The housekeeping <italic>dld</italic> gene was proposed in this study for detecting all mycoplasmas belonging to the '<italic>M.</italic> <italic>mycoides</italic>' cluster.</p> </list-item> <list-item id="u0015"> <label></label> <p id="p0015"> <italic>Mycoplasma mycoides</italic> subsp. <italic>capri</italic> (Mmc) was differentiated from <italic>Mycoplasma capricolum</italic> subsp. <italic>capricolum</italic> (Mcc) by PCR-RFLP.</p> </list-item> <list-item id="u0020"> <label></label> <p id="p0020">A new single tube PCR was developed in this study.</p> </list-item> <list-item id="u0025"> <label></label> <p id="p0025">PCR-RFLP and PCR provide rapid and useful assays in routine diagnosis.</p> </list-item> </list> </p> </sec> </abstract>