DNA barcoding of Bradysia (Diptera: Sciaridae) for detection of the immature stages on agricultural crops. (29th December 2014)

• Record Type: Journal Article
• Authors: Shin, S.; Jung, S.; Heller, K.; Menzel, F.; Hong, T. K.; Shin, J. S.; Lee, S. H.; Lee, H.; Lee, S.
• Is Part Of: Journal of applied entomology. Volume 139:Number 8(2015)
• Page Start: 638
• Page End: 645
• Publication Date: 2014-12-29
• Subjects: Entomology -- Periodicals; Insect pests -- Periodicals; 595.7
• DOI: 10.1111/jen.12198
• Languages: English
• ISSNs: 0931-2048

Abstract

<abstract abstract-type="main" id="jen12198-abs-0001"> <title>Abstract</title> <p>We investigated the usefulness of mitochondrial cytochrome <italic>c</italic> oxidase (COI) DNA barcoding of the genus <italic>Bradysia</italic> for the detection of immature stages and cryptic species complex. Although the larvae of some species in this genus are agricultural pests, immature stages are rarely identified due to the lack of key morphological characteristics. We constructed partial sequences of the COI gene for 25 species of <italic>Bradysia</italic> as a first step towards a DNA barcode. Using these data, <italic>Bradysia impatiens</italic>, <italic> B</italic>.<italic> procera</italic> and <italic>B</italic>. <italic>peraffinis</italic> were identified from larval specimens collected, respectively, from paprika, ginseng and oak sawdust beds used for cultivating shiitake. Our findings reveal a complex of three species within the <italic>B</italic>. <italic>tilicola</italic> group. These species were all identified as important pest <italic>B</italic>. <italic>ocellaris</italic> based on the morphology of male genital structures; however, the interspecific genetic divergence of the COI region was significantly greater (16.1–19.4%) than the intraspecific variation in each species. Therefore, <italic>B</italic>. <italic>ocellaris</italic> may consist of at least three species. The results demonstrate that COI DNA barcodes are useful for <italic>Bradysia</italic> species identification.</p> </abstract>