Identification of two arginine kinase forms of endoparasitoid Leptomastix dactylopii venom by bottom up‐sequence tag approach. (7th April 2015)

• Record Type: Journal Article
• Authors: Labella, Cristiana; Kanawati, Basem; Vogel, Heiko; Schmitt‐Kopplin, Philippe; Laurino, Simona; Bianco, Giuliana; Falabella, Patrizia
• Is Part Of: Journal of mass spectrometry. Volume 50:Number 5(2015:May)
• Page Start: 756
• Page End: 765
• Publication Date: 2015-04-07
• Subjects: Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/jms.3585
• Languages: English
• ISSNs: 1076-5174

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Leptomastix dactylopii</italic> (Howard) is an endoparasitoid wasp, natural enemy of mealybug <italic>Planococcus citri</italic> (Risso). Despite the acquired knowledge regarding this host‐parasitoid interaction, only little information is available on the factors of parasitoid origin able to modulate the mealybug physiology. The major alteration observed in <italic>P</italic>. <italic>citri</italic> is a strong reduction in fecundity, which is evident soon after parasitization by <italic>L</italic>. <italic>dactylopii</italic> or venom injection in unparasitized hosts indicating that this proteinaceus secretion injected at the oviposition plays a key‐role in host regulation. Protein identification of <italic>L</italic>. <italic>dactilopii</italic> venom has been limited by the lack of literature sources and public protein databases. Here, we identified two venom proteins by an integrated trascriptomic and proteomic approach. A custom‐made transcriptomic database from the <italic>L</italic>. <italic>dactylopii</italic> venom glands was created by applying the high‐throughput RNA sequencing approach. Two‐dimensional gel electrophoresis (2DE) trypsinized protein spots were analyzed by high‐resolution mass spectrometry (FTICRMS‐12 T). The most abundant peptide ions were fragmented by collision induced dissociation and the obtained sequence tags were subjected to custom‐made protein database searching. Two putative arginine kinases (full‐length and truncated form) were identified. This is the first case in which both, truncated and full length arginine kinases, are identified in an endoparasitoid non‐paralyzing venom. Copyright © 2015 John Wiley & Sons, Ltd.</p> </abstract>