MUC1/SEC and MUC1/Y overexpression is associated with inflammation in Sjögren's syndrome. (6th April 2015)

• Record Type: Journal Article
• Authors: Sung, HH; Castro, I; González, S; Aguilera, S; Smorodinsky, NI; Quest, AFG; Bahamondes, V; Alliende, C; Cortés, J; Molina, C; Urzúa, U; Barrera, M‐J; Hermoso, M; Herrera, L; Leyton, C; González, M‐J
• Is Part Of: Oral diseases. Volume 21:Number 6(2015:Oct.)
• Page Start: 730
• Page End: 738
• Publication Date: 2015-04-06
• Subjects: Mouth -- Diseases -- Research -- Periodicals; 617.522
• DOI: 10.1111/odi.12339
• Languages: English
• ISSNs: 1354-523X

Abstract

<abstract abstract-type="main" id="odi12339-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="odi12339-sec-0001" sec-type="section"> <title>Objectives</title> <p>To evaluate the expression and localization of MUC1/SEC and MUC1/Y isoforms in labial salivary glands (LSG) from Sjögren's syndrome patients (SS patients), as well as their <italic>in vitro</italic> expression induced by cytokines.</p> </sec> <sec id="odi12339-sec-0002" sec-type="section"> <title>Subjects and methods</title> <p>Labial salivary gland from 27 primary SS patients and 22 non‐SS sicca subjects were studied. Relative MUC1/SEC and MUC1/Y mRNA levels were determined by qPCR and protein levels by Western blotting. Induction of mucin mRNAs was assayed <italic>in vitro</italic>. Immunohistochemistry was used for localization.</p> </sec> <sec id="odi12339-sec-0003" sec-type="section"> <title>Results</title> <p>Relative MUC1/SEC and MUC1/Y mRNA and protein levels were significantly higher in LSG from SS patients. These mRNAs were induced by cytokines. MUC1/SEC and MUC1/Y were detected in acini apical region of control LSGs, and significant cytoplasmic accumulation was observed in acini of SS patients. MUC1/Y localized in acinar nuclei and cytoplasm of inflammatory cells of LSG from SS patients. A strong positive correlation was observed between cellular MUC1/SEC levels and glandular function determined by scintigraphy.</p> </sec> <sec id="odi12339-sec-0004" sec-type="section"> <title>Conclusions</title> <p>We show for the first time that MUC1/SEC and MUC1/Y are expressed in LSG of both SS patients and non‐SS sicca subjects. The observed overexpression and aberrant localization of MUC1/SEC and MUC1/Y and their induction by pro‐inflammatory cytokines may favor the perpetuation of the inflammatory environment that disrupts the salivary glandular homeostasis in SS patients.</p> </sec> </abstract>