Mutagenicity monitoring following battlefield exposures: Molecular analysis of HPRT mutations in gulf war i veterans exposed to depleted uranium. (12th May 2015)

• Record Type: Journal Article
• Authors: Nicklas, Janice A.; Albertini, Richard J.; Vacek, Pamela M.; Ardell, Stephanie K.; Carter, Elizabeth W.; McDiarmid, Melissa A.; Engelhardt, Susan M.; Gucer, Patricia W.; Squibb, Katherine S.
• Is Part Of: Environmental and molecular mutagenesis. Volume 56:Number 7(2015:Aug.)
• Page Start: 594
• Page End: 608
• Publication Date: 2015-05-12
• Subjects: Mutagenesis -- Periodicals; Molecular genetics -- Periodicals; Mutagenèse -- Périodiques; Mutagenèse chimique -- Périodiques; Mutation -- Périodiques; Maladies de l'environnement -- Périodiques; Génétique moléculaire -- Périodiques; 576.542
• DOI: 10.1002/em.21956
• Languages: English
• ISSNs: 0893-6692

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Molecular studies that involved cDNA and genomic DNA sequencing as well as multiplex PCR of the <italic>HPRT</italic> gene were performed to determine the molecular mutational spectrum for 1, 377 <italic>HPRT</italic> mutant isolates obtained from 61 Veterans of the 1991 Gulf War, most of whom were exposed to depleted uranium (DU). Mutant colonies were isolated from one to four times from each Veteran (in 2003, 2005, 2007, and/or 2009). The relative frequencies of the various types of mutations (point mutations, deletions, insertions, etc.) were compared between high versus low DU exposed groups, (based on their urine U concentration levels), with <italic>HPRT</italic> mutant frequency (as determined in the companion paper) and with a database of historic controls. The mutational spectrum includes all classes of gene mutations with no significant differences observed in Veterans related to their DU exposures. Environ. Mol. Mutagen. 56:594–608, 2015. © 2015 Wiley Periodicals, Inc.</p> </abstract>