Crystallographic studies of the extracytoplasmic function σ factor σJ from Mycobacterium tuberculosis. Issue 8 (1st August 2015)

• Record Type: Journal Article
• Authors: Goutam, Kapil; Gupta, Arvind Kumar; Gopal, Balasubramanian
• Is Part Of: Acta crystallographica. Volume 71:Issue 8(2015:Aug.)
• Page Start: 946
• Page End: 950
• Publication Date: 2015-08-01
• Subjects: Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X15009577
• Languages: English
• ISSNs: 2053-230X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Mycobacterium tuberculosis</italic> has multiple σ factors which enable the bacterium to reprogram its transcriptional machinery under diverse environmental conditions. σ^J, an extracytoplasmic function σ factor, is upregulated in late stationary phase cultures and during human macrophage infection. σ^J governs the cellular response to hydrogen peroxide‐mediated oxidative stress. σ^J differs from other canonical σ factors owing to the presence of a SnoaL_2 domain at the C‐terminus. σ^J crystals belonged to the tetragonal space group <italic>I</italic>422, with unit‐cell parameters <italic>a</italic> = <italic>b</italic> = 133.85, <italic>c</italic> = 75.08 Å. Diffraction data were collected to 2.16 Å resolution on the BM14 beamline at the European Synchrotron Radiation Facility (ESRF).</p> </abstract>