Analyzing the higher order structure of proteins with conformer‐selective ultraviolet photodissociation. Issue 16 (27th April 2015)

• Record Type: Journal Article
• Authors: Warnke, Stephan; von Helden, Gert; Pagel, Kevin; Barran, Perdita; Cooper, Helen; Eyers, Claire
• Is Part Of: Proteomics. Volume 15:Issue 16(2015:Aug.)
• Page Start: 2804
• Page End: 2812
• Publication Date: 2015-04-27
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201400480
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The top‐down approach in protein sequencing requires simple methods in which the analyte can be readily dissociated at every position along the backbone. In this context, ultraviolet photodissociation (UVPD) recently emerged as a promising tool because, in contrast to slow heating techniques such as CID, the absorption of UV light is followed by a rather statistically distributed cleavage of backbone bonds. As a result, nearly complete sequence coverage can be obtained. It is well known, however, that gas‐phase proteins can adopt a variety of different, sometimes coexisting conformations and the influence of this structural diversity on the UVPD fragmentation behavior is not clear. Using ion mobility‐UVPD‐MS, we recently showed that UVPD is sensitive to the higher order structure of gas‐phase proteins. In particular, the <italic>cis/trans</italic> isomerization of certain proline peptide bonds was shown to significantly influence the UVPD fragmentation pattern of two extended conformers of 11<sup>+</sup> ubiquitin. Building on these results, we here provide conformer‐selective UVPD data for 7<sup>+</sup> ubiquitin ions, which are known to be present in a much more diverse and wider ensemble of different structures, ranging from very compact to highly extended species. Our data show that certain conformers fall into groups with similar UVPD fragmentation pattern. Surprisingly, however, the conformers within each group can differ tremendously in their collision cross‐section. This indicates that the multiple coexisting conformations typically observed for 7<sup>+</sup> ubiquitin are caused by a few, not easily interconvertible, subpopulations.</p> </abstract>