Cytotoxicity of arctigenin and matairesinol against the T‐cell lymphoma cell line CCRF‐CEM. (29th April 2015)

• Record Type: Journal Article
• Authors: Su, Shan; Cheng, Xinlai; Wink, Michael
• Is Part Of: Journal of pharmacy and pharmacology. Volume 67:Number 9(2015:Sep.)
• Page Start: 1316
• Page End: 1323
• Publication Date: 2015-04-29
• Subjects: Pharmacy -- Periodicals; Pharmacology -- Periodicals; 615.1
• DOI: 10.1111/jphp.12426
• Languages: English
• ISSNs: 0022-3573

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="jphp12426-sec-0001" sec-type="section"> <title>Objectives</title> <p>Arctigenin and matairesinol possess a diversity of bioactivities. Here we investigated the cytotoxicity of arctigenin and matairesinol against a T‐cell lymphoma cell line CCRF‐CEM and the underlying mechanisms that have not been explored before.</p> </sec> <sec id="jphp12426-sec-0002" sec-type="section"> <title>Methods</title> <p>The cytotoxic activity was investigated using MTT assay. The cell cycle arrest and reactive oxygen species (ROS) accumulation were determined by flow cytometric analysis. The apoptosis induction was assessed using Annexin V/Propidium Iodide assay. The gene quantification analysis was measured through real‐time polymerase chain reaction.</p> </sec> <sec id="jphp12426-sec-0003" sec-type="section"> <title>Key findings</title> <p>Arctigenin and matairesinol exhibited significant antiproliferative activity against CCRF‐CEM cells after 72 h treatment with IC50 values of 1.21 ± 0.15 μ<sc>m</sc> and 4.27 ± 0.41 μ<sc>m</sc>, respectively. In addition, both lignans arrest CCRF‐CEM cells in the S phase. Furthermore, they could induce apoptosis in CCRF‐CEM cells in a concentration‐ and time‐dependent manner. Interestingly, the lignans differentially regulated the expression of several key genes involved in apoptosis pathways, including <italic>B</italic><italic>ax</italic>, <italic>B</italic><italic>ad</italic> and <italic>caspase‐9</italic>. Moreover, both lignans could increase ROS levels in CCRF‐CEM cells.</p> </sec> <sec id="jphp12426-sec-0004" sec-type="section"> <title>Conclusions</title> <p>Our study provides an insight into the potential of arctigenin and matairesinol as good candidates for the development of novel agents against T‐cell lymphoma.</p> </sec> </abstract>