Co‐expression of march5b and tlr7 in large yellow croaker Larimichthys crocea in response to Cryptocaryon irritans infection. Issue 2 (14th July 2015)

• Record Type: Journal Article
• Authors: Zhang, D. L.; Yu, D. H.; Chen, J.; Chen, C.; Wang, Z. Y.
• Is Part Of: Journal of fish biology. Volume 87:Issue 2(2015)
• Page Start: 360
• Page End: 370
• Publication Date: 2015-07-14
• Subjects: Fishes -- Periodicals; Fishes -- Great Britain -- Periodicals; 597
• DOI: 10.1111/jfb.12726
• Languages: English
• ISSNs: 0022-1112

Abstract

<abstract abstract-type="main" id="jfb12726-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="jfb12726-para-0001">In this study, molecular characteristics of <italic>march5b</italic> and co‐expression of <italic>march5b</italic> and <italic>tlr7</italic> in response to the infection of <italic>Cryptocaryon irritans</italic> in the large yellow croaker <italic>Larimichthys crocea</italic> were investigated. The full‐length complementary (c)DNA of <italic>march5b</italic> was 1314 bp, including an open reading frame of 846 bp encoding a polypeptide of 281 amino acids, and the full‐length genomic sequence was composed of 23 577 nucleotides, including six exons and five introns. The putative March5b protein contained a RINGv motif and four transmembrane domains. The <italic>march5b</italic> transcripts were broadly distributed in all detected tissues, with a strong expression in blood, brain and gills, and a weak expression in kidney by quantitative PCR analysis. The expression of <italic>march5b</italic> and <italic>tlr7</italic> in the skin, gills, spleen and head kidney changed in the same manner at most time points post‐primary infection with <italic>C. irritans</italic>. Significant increase was observed in the skin with <italic>march5b</italic> at days 2 and 3 by 26·10 and 6·88 fold, respectively, and with <italic>tlr7</italic> at day 3 by 57·68 fold, when compared with the control. Their expressions, however, were decreased in the gills, especially at day 3 (<italic>march5b</italic> by 8·9%, <italic>tlr7</italic> by 22·06%). In the spleen and head kidney, <italic>march5b</italic> and <italic>tlr7</italic> transcripts were up‐regulated early, then noticeably declined at day 3. These results suggested that <italic>march5b</italic> and <italic>tlr7</italic> are co‐expressed in response to parasite infection and March5b probably catalyses ubiquitination of some proteins of TLR7 signalling pathway.</p> </abstract>