Enhanced differentiation of intraepithelial lymphocytes in the intestine of polymeric immunoglobulin receptor‐deficient mice. Issue 1 (26th June 2015)

• Record Type: Journal Article
• Authors: Kato‐Nagaoka, Noriko; Shimada, Shin‐Ichiro; Yamakawa, Yoko; Tsujibe, Satoshi; Naito, Tomoaki; Setoyama, Hiromi; Watanabe, Yohei; Shida, Kan; Matsumoto, Satoshi; Nanno, Masanobu
• Is Part Of: Immunology. Volume 146:Issue 1(2015:Sep.)
• Page Start: 59
• Page End: 69
• Publication Date: 2015-06-26
• Subjects: Immunology -- Periodicals
• DOI: 10.1111/imm.12480
• Languages: English
• ISSNs: 0019-2805

Abstract

<abstract abstract-type="main" id="imm12480-abs-0001"> <title>Summary</title> <p>To clarify the effect of secretory IgA (sIgA) deficiency on gut homeostasis, we examined intraepithelial lymphocytes (IELs) in the small intestine (SI) of polymeric immunoglobulin receptor‐deficient (pIgR<sup>−/−</sup>) mice. The pIgR<sup>−/−</sup> mice exhibited the accumulation of CD8<italic>αβ</italic><sup>+</sup> T‐cell receptor (TCR)‐<italic>αβ</italic><sup>+</sup> IELs (CD8<italic>αβ</italic><sup>+</sup><italic>αβ</italic>‐IELs) after weaning, but no increase of CD8<italic>αβ</italic><sup>+</sup><italic>γδ</italic>‐IELs was detected in pIgR<sup>−/−</sup> TCR‐<italic>β</italic><sup>−/−</sup> mice compared with pIgR<sup>+/+</sup> TCR‐<italic>β</italic><sup>−/−</sup> mice. When 5‐bromo‐2′‐deoxyuridine (BrdU) was given for 14 days, the proportion of BrdU‐labelled cells in SI‐IELs was not different between pIgR<sup>+/+</sup> mice and pIgR<sup>−/−</sup> mice. However, the proportion of BrdU‐labelled CD8<italic>αβ</italic><sup>+</sup>‐IELs became higher in pIgR<sup>−/−</sup> mice than pIgR<sup>+/+</sup> mice 10 days after discontinuing BrdU‐labelling. Intravenously transferred splenic T cells migrated into the intraepithelial compartments of pIgR<sup>+/+</sup> TCR‐<italic>β</italic><sup>−/−</sup> mice and pIgR<sup>−/−</sup> TCR‐<italic>β</italic><sup>−/−</sup> mice to a similar extent. In contrast, in the case of injection of immature bone marrow cells, CD8<italic>αβ</italic><sup>+</sup><italic>αβ</italic>‐IELs increased much more in the SI of pIgR<sup>−/−</sup> TCR‐<italic>β</italic><sup>−/−</sup> mice than pIgR<sup>+/+</sup> TCR‐<italic>β</italic><sup>−/−</sup> mice 8 weeks after the transfer. <italic>αβ</italic>‐IELs from pIgR<sup>−/−</sup> mice could produce more interferon‐<italic>γ</italic> and interleukin‐17 than those of pIgR<sup>+/+</sup> mice, and intestinal permeability tended to increase in the SI of pIgR<sup>−/−</sup> mice with aging. Taken together, these results indicate that activated CD8<italic>αβ</italic><sup>+</sup><italic>αβ</italic>‐IELs preferentially accumulate in pIgR<sup>−/−</sup> mice through the enhanced differentiation of immature haematopoietic precursor cells, which may subsequently result in the disruption of epithelial integrity.</p> </abstract>