Fine mapping of the genetic locus L1 conferring black pods using a chromosome segment substitution line population of soybean. Issue 4 (25th May 2015)

• Record Type: Journal Article
• Authors: He, Qingyuan; Yang, Hongyan; Xiang, Shihua; Tian, Dong; Wang, Wubin; Zhao, Tuanjie; Gai, Junyi; Singh, R.
• Is Part Of: Plant breeding. Volume 134:Issue 4(2015)
• Page Start: 437
• Page End: 445
• Publication Date: 2015-05-25
• Subjects: Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12272
• Languages: English
• ISSNs: 0179-9541

Abstract

<abstract abstract-type="main" id="pbr12272-abs-0001"> <title>Abstract</title> <p>The colour of plant organs is a useful trait in crop breeding. The pod colours of soybeans primarily include black, brown and tan types, which are controlled by two classical genetic loci, <italic>L1</italic> and <italic>L2</italic>. Most wild soybeans have black pods, which reflect a possible role in adaptation to the natural environment. Here, an improved chromosome segment substitution line (CSSL) population <italic>SojaCSSLP3</italic> was established to identify the <italic>L1</italic> gene. The segment on the 19th chromosome represented by the SSR marker Satt313 was found to link with locus <italic>L1</italic>. The region was further delimited three times with increased SSR and InDel markers using a population derived from a heterozygous plant of <italic>CSSL124</italic> from <italic>SojaCSSLP3</italic>. The <italic>L1</italic> gene was finally located in a 184.43‐kb region between SSR_19p09 and Indel_19P7. Thirteen putative genes in this region were analysed with <italic>q</italic>RT‐PCR. The expression level of Glyma19 g27460, which is a member of the SANT superfamily with a MYB DNA‐binding domain, was significantly upregulated in black pods and was recognized to be the most likely candidate for the <italic>L1</italic> gene.</p> </abstract>