Barley Ant1 is a homolog of maize C1 and its product is part of the regulatory machinery governing anthocyanin synthesis in the leaf sheath. Issue 4 (10th June 2015)

• Record Type: Journal Article
• Authors: Shoeva, Olesya Y.; Kukoeva, Tatyana V.; Börner, Andreas; Khlestkina, Elena K.; Saranga, Y.
• Is Part Of: Plant breeding. Volume 134:Issue 4(2015)
• Page Start: 400
• Page End: 405
• Publication Date: 2015-06-10
• Subjects: Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12277
• Languages: English
• ISSNs: 0179-9541

Abstract

<abstract abstract-type="main" id="pbr12277-abs-0001"> <title>Abstract</title> <p>Anthocyanins contribute to plants' defence against a number of abiotic and biotic stress agents. The anthocyanin pigmentation of the barley leaf sheath is genetically determined by <italic>Ant1, </italic> a gene which maps to a region of chromosome 7HS delimited by the microsatellite loci <italic>Xgbms0226</italic> and <italic>Xgbms0240</italic>. The sequence of the maize gene <italic>C1</italic> (encoding an R2R3 MYB factor regulating anthocyanin synthesis) was used for the PCR‐based cloning of <italic>Ant1</italic>. In <italic>ant1</italic> genotypes, no transcript is generated in the leaf sheath, whereas the gene is active in the presence of the dominant allele. A comparison of the coding and promoter sequences of <italic>Ant1</italic> (which induces purple pigmentation in the leaf sheath) and <italic>ant1</italic> (which does not) showed that the key polymorphisms lay in the promoter sequence. The transcription of four anthocyanin synthesis structural genes (<italic>Chi</italic>, <italic> F3h</italic>, <italic> Dfr</italic>, <italic> Ans</italic>) was dependent on the allelic status of <italic>Ant1</italic>.</p> </abstract>