A rapid and sensitive LC‐MS/MS method for quantification of 3, 29‐dibenzoyl rarounitriol in rat plasma: application to a pharmacokinetic study. (22nd January 2015)

• Record Type: Journal Article
• Authors: Zhao, Chengliang; Zhang, Nan; Chen, Bin; Song, Youxin; Zhu, Naiqiang; Zhao, Long; Liu, Changjian; Meng, Xiangwei
• Is Part Of: Biomedical chromatography. Volume 29:Number 8(2015:Aug.)
• Page Start: 1229
• Page End: 1234
• Publication Date: 2015-01-22
• Subjects: Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3412
• Languages: English
• ISSNs: 0269-3879

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>A rapid, sensitive and high‐throughput liquid chromatography–tandem mass spectrometry was established and validated to assay the concentrations of 3, 29‐dibenzoyl rarounitriol in rat plasma. Plasma samples were processed by liquid–liquid extraction with ethyl acetate and separated on a Hypersil Gold C₁₈ column (50 × 4.6 mm, 3 µm) at an isocratic flow rate of 0.5 mL/min using methanol–10 m<sc>m</sc> ammonium acetate–formic acid (90:10:0.1, v/v/v) as mobile phase. The total run time was 5 min for each sample. MS/MS detection was accomplished in selected reaction monitoring mode with positive electrospray ionization. The calibration curve was linear over the concentration range of 0.125–50 ng/mL with lower limit of quantification of 0.125 ng/mL. The intra‐ and inter‐day precisions were &lt;10.1% in terms of coefficient of variation, and the accuracy was within ±11.7% in terms of relative error. The developed method was successfully applied to a pharmacokinetic study of 3, 29‐dibenzoyl rarounitriol following intragastric administration of 3.65 mg/kg to Wistar rats. Copyright © 2015 John Wiley &amp; Sons, Ltd.</p> </abstract>