Treatment with a neutralising anti-rat interleukin-17 antibody after multiple-trauma reduces lung inflammation. Issue 8 (August 2015)

• Record Type: Journal Article
• Authors: Dai, Heling; Xu, Li; Tang, Yu; Liu, Zhi; Sun, Tiansheng
• Is Part Of: Injury. Volume 46:Issue 8(2015)
• Page Start: 1465
• Page End: 1470
• Publication Date: 2015-08
• Subjects: Wounds and injuries -- Surgery -- Periodicals; Accidents -- Periodicals; Wounds and Injuries -- surgery -- Periodicals; Lésions et blessures -- Chirurgie -- Périodiques; Electronic journals; Electronic journals; 617.1
• DOI: 10.1016/j.injury.2015.05.016
• Languages: English
• ISSNs: 0020-1383

Abstract

<abstract abstract-type="author" id="abs0005"> <title id="sect0005">Abstract</title> <sec> <title id="sect0010">Background</title> <p id="spar0005">It has been well recognised that a deficit of numbers and function of CD4<sup>+</sup>CD25<sup>+</sup>Foxp3<sup>+</sup> cells (Treg) is attributed to the development of autoimmune diseases and inflammatory diseases; additionally, IL-17-producing cells (Th17) have a pro-inflammatory role. The balance between Th17 and Treg may be essential for maintaining immune homeostasis and has long been thought as one of the important factors in the development/prevention of autoimmune diseases and inflammatory diseases. In our previous research, we explored that cytokines (IL-17) and the balance of Treg/Th17 had a significant relevance with tissue (lung) inflammation and injury in acute-phase after multiple-trauma.</p> </sec> <sec> <title id="sect0015">Objective</title> <p id="spar0010">To more verify whether an imbalance of Treg/Th17 is characteristic of rats suffering from multiple trauma.</p> </sec> <sec> <title id="sect0020">Methods and subjective</title> <p id="spar0015">Using IL-17 monoclonal antibody (IL-17mAb)-treated multiple-trauma rat, we tested the pathogenic role of IL-17 in the development of multiple-trauma. Rat models were treated respectively with IL-17mAb or rat IgG 2A isotype control or phosphate-buffered solution after model was established. Normal rats only received anaesthesia and cannulation were taken as sham. Rats in each group were killed respectively at the end of 1 h, 4 h, 8 h after injection. Collected serum and lung samples for assessment dynamically of MPO, IL-17, IL-6, and TGF-β-mRNA, and cytokine (IL-17, IL-6, TGF-β) and lung tissue for pulmonary histological analysis.</p> </sec> <sec> <title id="sect0025">Results</title> <p id="spar0020">Neutralisation of IL-17 with anti-IL-17 can decrease serum IL-17 level and the IL-17-mRNA transcript level in lung, and ameliorate tissue inflammatory, defer disease course.</p> </sec> <sec> <title id="sect0030">Conclusion</title> <p id="spar0025">Our data suggest that IL-17 is crucially involved in the pathogenesis of multiple-trauma in rat, IL-17 inhibition might ameliorate the lung inflammation in acute-phase after multiple-trauma.</p> </sec> </abstract>