High-resolution probing heparan sulfate–antithrombin interaction on a single endothelial cell surface: single-molecule AFM studies1. Issue 20 (28th May 2015)

• Record Type: Journal Article
• Authors: Guo, Cunlan; Fan, Xian; Qiu, Hong; Xiao, Wenyuan; Wang, Lianchun; Xu, Bingqian
• Is Part Of: Physical chemistry chemical physics. Volume 17:Issue 20(2015)
• Page Start: 13301
• Page End: 13306
• Publication Date: 2015-05-28
• Subjects: Chemistry, Physical and theoretical -- Periodicals; 541.3
• DOI: 10.1039/c5cp01305d
• Languages: English
• ISSNs: 1463-9076

Abstract

<abstract abstract-type="toc"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <graphic position="anchor" id="ga" xlink:href="ark:/27927/pgj14jc7ds9" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" />Using an antithrombin functionalized AFM probe, the specific interactions between antithrombin and heparan sulfate on endothelial cell surface were determined, revealing previously unexpected diverse binding sites as well as critical roles of <italic>N</italic>-, 2-<italic>O</italic>- and 6-<italic>O</italic>-sulfation in the interactions.</p> </abstract>