Correlation between the dielectric properties and biological activities of human ex vivo hepatic tissue. (21st March 2015)

• Record Type: Journal Article
• Authors: Wang, Hang; He, Yong; Yan, Qingguo; You, Fusheng; Fu, Feng; Dong, Xiuzhen; Shi, Xuetao; Yang, Min
• Is Part Of: Physics in medicine & biology. Volume 60:Number 6(2015:Mar.)
• Page Start: 2603
• Page End: 2617
• Publication Date: 2015-03-21
• Subjects: Biophysics -- Periodicals; Medical physics -- Periodicals; 610.153
• DOI: 10.1088/0031-9155/60/6/2603
• Languages: English
• ISSNs: 0031-9155

Abstract

<abstract> <title>Abstract</title> <p>Dielectric properties are vital biophysical features of biological tissues, and biological activity is an index to ascertain the active state of tissues. This study investigated the potential correlation between the dielectric properties and biological activities of human hepatic tissue with prolonged <italic>ex vivo</italic> time through correlation and regression analyses. The dielectric properties of 26 cases of normal human hepatic tissue at 10 Hz to 100 MHz were measured from 15 min after isolation to 24 h at 37 °C with 90% humidity. Cell morphologies, including nucleus area (NA) and alteration rate of intercellular area (ICAR), were analyzed as indicators of biological activities. Conductivity, complex resistivity, and NA exhibited opposing changes 1 h after isolation. Relative permittivity and <italic>ex vivo</italic> time were not closely correlated (<italic>p</italic> > 0.05). The dielectric properties measured at low frequencies (i.e. <1 MHz) were more sensitive than those measured at high frequencies in reflecting the biological activity of <italic>ex vivo</italic> tissue. Highly significant correlations were found between conductivity, resistivity and the <italic>ex vivo</italic> time (<italic>p</italic> < 0.05) as well as conductivity and the cell morphology (<italic>p</italic> < 0.05). The findings indicated that establishing the correlation between the dielectric properties and biological activities of human hepatic tissue is of great significance for promoting the role of dielectric properties in biological science, particularly in human biology.</p> </abstract>