99mTc-labeled porphyrin–lipid nanovesicles. (June 2015)

• Record Type: Journal Article
• Authors: Lee, Jae-Ho; Shao, Shuai; Cheng, Kenneth T.; Lovell, Jonathan F.; Paik, Chang H.
• Is Part Of: Journal of liposome research. Volume 25:Number 2(2015)
• Page Start: 101
• Page End: 106
• Publication Date: 2015-06
• Subjects: Liposomes -- Periodicals; Liposomes -- Periodicals; 575.57
• Languages: English
• ISSNs: 0898-2104

Abstract

<abstract> <title>Abstract</title> <p>Porphyrin–lipid nanovesicles (PLN) have been developed with intrinsic capabilities as activatable multimodal photonic contrast agents. Radiolabeling of PLN encapsulating drugs could eventually be able to provide quantitative <italic>in vivo</italic> information for diagnosing and treating diseases. In this study, we developed ^99mTc-labeled porphyrin–lipid nanovesicles (^99mTc-PLN) as a cargo-encapsulated formulation without significant impact on liposome integrity and encapsulation stability. 50 mM calcein was encapsulated into PLN by probe sonication. The size of the PLN was about 150 nm. The PLN were then reacted with ^99mTc using SnCl₂ dissolved in 1 mM HCl as a reducing agent and incubated for 10 min at 22 °C. The radiolabeling efficiency and stability of ^99mTc-PLN were evaluated by instant thin-layer chromatography and low-pressure liquid chromatography (LPLC). ^99mTc labeling was successful with a &gt;92% labeling efficiency. LPLC showed that the liposomal elution peaks of the porphyrin–lipid and the calcein overlapped with the radioactivity elution peak of ^99mTc-labeled PLN. The ^99mTc-labeling procedure did not change the size of PLN. Encapsulated calcein remained inert inside PLN. Thus, this work lays out a simple and effective radiolabeling method using SnCl₂ in HCl in the preparation of ^99mTc-PLN.</p> </abstract>