Genetic characterization of Moniezia species in Senegal and Ethiopia. Issue 5 (October 2015)

• Record Type: Journal Article
• Authors: Diop, Gora; Yanagida, Tetsuya; Hailemariam, Zerihun; Menkir, Sissay; Nakao, Minoru; Sako, Yasuhito; Ba, Cheikh Tidiane; Ito, Akira
• Is Part Of: Parasitology international. Volume 64:Issue 5(2015:Oct.)
• Page Start: 256
• Page End: 260
• Publication Date: 2015-10
• Subjects: Parasitology -- Periodicals; Parasites -- Periodicals; Parasitic Diseases -- Periodicals; Parasitology -- Periodicals; Parasitologie -- Périodiques; 571.99905
• DOI: 10.1016/j.parint.2015.02.008
• Languages: English
• ISSNs: 1383-5769

Abstract

<abstract abstract-type="author" id="ab0005"> <title id="st0005">Abstract</title> <sec> <p id="sp0005">Genetic diversity of <italic>Moniezia</italic> spp. from domestic ruminants in Senegal and Ethiopia was investigated based on the nucleotide sequences of mitochondrial cytochrome <italic>c</italic> oxidase subunit 1 (<italic>cox1</italic>) and nuclear small subunit ribosomal RNA gene (SSU rDNA). A total of 64 adult tapeworms were collected from sheep, goat and cattle, and the tapeworms from cattle were all morphologically identified as <italic>Moniezia benedeni</italic>. On the other hand, the tapeworms obtained from sheep and goat were identified as <italic>Moniezia expansa</italic> or could not be identified because of the lack of diagnostic morphologic character, i.e. interproglottidal glands (IPGs). Phylogenetic analysis based on <italic>cox1</italic> gene sequences revealed that the worms from sheep/goat and cattle formed distinct clades, and three mitochondrial lineages were confirmed within the sheep/goat tapeworms. The maximum pairwise divergences among the three mitochondrial linages were about 3% in <italic>cox1</italic> and 0.1% in SSU rDNA, while that between the worms from sheep/goat and cattle reached 13% in <italic>cox1</italic> and 2.7% in SSU rDNA. All of the three mitochondrial lineages contained tapeworms morphologically identified as <italic>M. expansa</italic>, and the tapeworms without IPGs were confirmed in one of the three lineages, indicating the tapeworms without IPGs were also <italic>M. expansa</italic>.</p> </sec> </abstract>