Establishment and characterization of a fish‐cell line from the brain of Japanese flounder Paralichthys olivaceus. Issue 1 (14th May 2015)

• Record Type: Journal Article
• Authors: Zheng, Y.; Peng, L. M.; You, F.; Zou, Y. X.; Zhang, P. J.; Chen, S. L.
• Is Part Of: Journal of fish biology. Volume 87:Issue 1(2015)
• Page Start: 115
• Page End: 122
• Publication Date: 2015-05-14
• Subjects: Fishes -- Periodicals; Fishes -- Great Britain -- Periodicals; 597
• DOI: 10.1111/jfb.12700
• Languages: English
• ISSNs: 0022-1112

Abstract

<abstract abstract-type="main" id="jfb12700-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="jfb12700-para-0001">A new brain‐cell line derived from Japanese flounder <italic>Paralichthys olivaceus</italic> (POBC) was established. POBC was subcultured for 67 passages over the course of 420 days. The cultured cells were primarily epithelioid‐like. Chromosome analysis revealed the cell line to possess the normal <italic>P. olivaceus</italic> diploid karyotype of 2n = 48t (telocentric chromosomes). The cells exhibited the astrocyte marker glial fibrillary acidic protein by immunocytochemistry, and significant fluorescent signals were observed when the cells were transfected with green fluorescent protein reporter plasmid. The established POBC would be ideal material for the study of function of fish ependyma, the central neuroendocrine system and endocrine disruptors in the marine environment.</p> </abstract>