A Smart DNAzyme–MnO2 Nanosystem for Efficient Gene Silencing1. Issue 16 (26th February 2015)

• Record Type: Journal Article
• Authors: Fan, Huanhuan; Zhao, Zilong; Yan, Guobei; Zhang, Xiaobing; Yang, Chao; Meng, Hongmin; Chen, Zhuo; Liu, Hui; Tan, Weihong
• Is Part Of: Angewandte Chemie international edition. Volume 54:Issue 16(2015)
• Page Start: 4801
• Page End: 4805
• Publication Date: 2015-02-26
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201411417
• Languages: English
• ISSNs: 1433-7851

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>DNAzymes hold promise for gene‐silencing therapy, but the lack of sufficient cofactors in the cell cytoplasm, poor membrane permeability, and poor biostability have limited the use of DNAzymes in therapeutics. We report a DNAzyme–MnO₂ nanosystem for gene‐silencing therapy. MnO₂ nanosheets adsorb chlorin e6‐labelled DNAzymes (Ce6), protect them from enzymatic digestion, and efficiently deliver them into cells. The nanosystem can also inhibit ¹O₂ generation by Ce6 in the circulatory system. In the presence of intracellular glutathione (GSH), MnO₂ is reduced to Mn²⁺ ions, which serve as cofactors of 10–23 DNAzyme for gene silencing. The release of Ce6 generates ¹O₂ for more efficient photodynamic therapy. The Mn²⁺ ions also enhance magnetic resonance contrast, providing GSH‐activated magnetic resonance imaging (MRI) of tumor cells. The integration of fluorescence recovery and MRI activation provides fluorescence/MRI bimodality for monitoring the delivery of DNAzymes.</p> </abstract>