The immunoglobulin M-degrading enzyme of Streptococcus suis, IdeSsuis, is a highly protective antigen against serotype 2. Issue 19 (5th May 2015)

• Record Type: Journal Article
• Authors: Seele, Jana; Hillermann, Lena-Maria; Beineke, Andreas; Seitz, Maren; von Pawel-Rammingen, Ulrich; Valentin-Weigand, Peter; Baums, Christoph G.
• Is Part Of: Vaccine. Volume 33:Issue 19(2015)
• Page Start: 2207
• Page End: 2212
• Publication Date: 2015-05-05
• Subjects: Vaccines -- Periodicals; 615.372
• DOI: 10.1016/j.vaccine.2015.03.047
• Languages: English
• ISSNs: 0264-410X

Abstract

<abstract abstract-type="author" id="abs0005"> <title id="sect0005">Abstract</title> <sec> <p id="spar0005"> <italic>Streptococcus suis</italic> (<italic>S. suis</italic>) is a major porcine pathogen causing meningitis, arthritis and several other pathologies. Recently, we identified a highly specific immunoglobulin M degrading enzyme of <italic>S. suis</italic>, designated Ide<sub><italic>Ssuis</italic></sub>, which is expressed by various serotypes. The objective of this work was to access the immunogenicity and protective efficacy of a recombinant vaccine including Ide<sub><italic>Ssuis</italic></sub>. Vaccination with rIde<sub><italic>Ssuis</italic></sub> elicited antibodies efficiently neutralizing the IgM protease activity. Importantly, 18 piglets vaccinated with rIde<sub><italic>Ssuis</italic></sub> alone or in combination with bacterin priming were completely protected against mortality and severe morbidity after <italic>S. suis</italic> serotype 2 challenge. In contrast, 12 of the 17 piglets either treated with the placebo or primed with the bacterin only, succumbed to <italic>S. suis</italic> disease. Immunity against Ide<sub><italic>Ssuis</italic></sub> was associated with increased killing of <italic>S. suis</italic> wt in porcine blood <italic>ex vivo</italic> leading to a tenfold difference in the bacterial survival factor in blood of placebo-treated and rIde<sub><italic>Ssuis</italic></sub>-vaccinated piglets. In conclusion, the results of this study indicate that rIde<sub><italic>Ssuis</italic></sub> is a highly protective antigen in pigs.</p> </sec> </abstract>