Myeloperoxidase-deficient zebrafish show an augmented inflammatory response to challenge with Candida albicans. Issue 1 (May 2015)

• Record Type: Journal Article
• Authors: Wang, Kun; Fang, Xiao; Ma, Ning; Lin, Qing; Huang, Zhibin; Liu, Wei; Xu, Mengchang; Chen, Xiaohui; Zhang, Wenqing; Zhang, Yiyue
• Is Part Of: Fish & shellfish immunology. Volume 44:Issue 1(2015:May)
• Page Start: 109
• Page End: 116
• Publication Date: 2015-05
• Subjects: Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.01.038
• Languages: English
• ISSNs: 1050-4648

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010">Myeloperoxidase is a key component of neutrophil granules involved in killing engulfed microorganisms. We obtained a zebrafish mutant (<italic>smu681</italic>) lacking Sudan black staining by large-scale screening, which was a neutrophil-replete but myeloperoxidase-deficient mutant. When infiltrated with <italic>Candida albicans</italic>, <italic>smu681</italic> embryos and sibling embryos showed similar survival after infection. Proliferation of <italic>C. albicans</italic> was more rapid in <italic>smu681</italic> embryos than in sibling embryos, although it was eventually suppressed. In addition, the number of neutrophils accumulating at the site of infection was significantly larger in mutant embryos than in sibling embryos, and mutant embryos showed increased expression of several inflammatory cytokines after <italic>C. albicans</italic> infection. These findings indicate that myeloperoxidase deficiency alters the inflammatory response to fungal infection.</p> </sec> </abstract>