Use of HPLC/UPLC-spectrophotometry for detection of formazan in in vitro Reconstructed human Tissue (RhT)-based test methods employing the MTT-reduction assay to expand their applicability to strongly coloured test chemicals. Issue 4 (June 2015)

• Record Type: Journal Article
• Authors: Alépée, N.; Barroso, J.; De Smedt, A.; De Wever, B.; Hibatallah, J.; Klaric, M.; Mewes, K.R.; Millet, M.; Pfannenbecker, U.; Tailhardat, M.; Templier, M.; McNamee, P.
• Is Part Of: Toxicology in vitro. Volume 29:Issue 4(2015)
• Page Start: 741
• Page End: 761
• Publication Date: 2015-06
• Subjects: Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2015.02.005
• Languages: English
• ISSNs: 0887-2333

Abstract

<abstract xml:lang="en" abstract-type="author-highlights" id="ab005"> <title id="st080">Highlights</title> <sec> <p id="sp0005"> <list id="l0005"> <list-item id="o0005"> <label></label> <p id="p0325">Key qualification parameters defined for HPLC/UPLC-spectrophotometry systems.</p> </list-item> <list-item id="o0010"> <label></label> <p id="p0330">HPLC/UPLC-spectrophotometry for formazan detection is highly reproducible.</p> </list-item> <list-item id="o0015"> <label></label> <p id="p0335">High concordance of classification using OD and HPLC/UPLC-spectrophotometry.</p> </list-item> <list-item id="o0020"> <label></label> <p id="p0340">Applicable for highly coloured chemicals interfering with standard detection method.</p> </list-item> <list-item id="o0025"> <label></label> <p id="p0345">Applicable to <italic>in vitro</italic> RhT test methods for eye/skin irritation and skin corrosion.</p> </list-item> </list> </p> </sec> </abstract>