The utility of HepG2 cells to identify direct mitochondrial dysfunction in the absence of cell death. Issue 4 (June 2015)

• Record Type: Journal Article
• Authors: Kamalian, Laleh; Chadwick, Amy E.; Bayliss, Mark; French, Neil S.; Monshouwer, Mario; Snoeys, Jan; Park, B. Kevin
• Is Part Of: Toxicology in vitro. Volume 29:Issue 4(2015)
• Page Start: 732
• Page End: 740
• Publication Date: 2015-06
• Subjects: Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2015.02.011
• Languages: English
• ISSNs: 0887-2333

Abstract

<abstract xml:lang="en" abstract-type="author-highlights" id="ab005"> <title id="st095">Highlights</title> <sec> <p id="sp0005"> <list id="l0005"> <list-item id="o0005"> <label></label> <p id="p0120">Short substitution of glucose with galactose in HepG2 media reveals mito-toxicity.</p> </list-item> <list-item id="o0010"> <label></label> <p id="p0125">Parallel ATP/cytotoxicity measurement provides a mitochondrial functional endpoint.</p> </list-item> <list-item id="o0015"> <label></label> <p id="p0130">A sensitive, cost-effective and fast first tier mitochondrial screen is proposed.</p> </list-item> <list-item id="o0020"> <label></label> <p id="p0135">HepG2 cells are a better model than fresh human hepatocytes to define mito-toxicity.</p> </list-item> </list> </p> </sec> </abstract>