Establishment of a canine model of acute pulmonary embolism with definite right ventricular dysfunction through introduced autologous blood clots. Issue 4 (April 2015)

• Record Type: Journal Article
• Authors: Zhao, Lin-Bo; Jia, Zhen-Yu; Lu, Guang-Dong; Zhu, Yin-Su; Jing, Lei; Shi, Hai-Bin
• Is Part Of: Thrombosis research. Volume 135:Issue 4(2015)
• Page Start: 727
• Page End: 732
• Publication Date: 2015-04
• Subjects: Thrombosis -- Periodicals; 616.135
• DOI: 10.1016/j.thromres.2015.01.016
• Languages: English
• ISSNs: 0049-3848

Abstract

<abstract abstract-type="author" id="ab0005"> <title id="st0005">Abstract</title> <sec> <title id="st0010">Purpose</title> <p id="sp0005">To establish a canine model of acute pulmonary embolism (PE) with right ventricular (RV) dysfunction using autologous blood clots and evaluate by echocardiography and contrast-enhanced Computed Tomography (CT).</p> </sec> <sec> <title id="st0015">Materials and Methods</title> <p id="sp0010">Autologous blood clots formed <italic>in vitro</italic> were introduced sequentially into the pulmonary arteries of eight healthy mixed-breed dogs while monitoring pulmonary and systemic hemodynamic function. Blood clots were injected until the mean pulmonary artery pressure (MPAP) reached two–three times the baseline pressure, which was maintained up to 1 hour. The RV function was assessed by echocardiography and ECG-gated dual-source contrast CT.</p> </sec> <sec> <title id="st0020">Results</title> <p id="sp0015">All animals survived the imaging procedure. The post-injection pulmonary angiograms showed extensive PE, and MPAP increased from 16.50 ± 2.45 mmHg to 43.13 ± 4.91 mmHg (P < 0.001). On echocardiography, the RV fractional area change decreased from 42.06 ± 3.36 to 27.96 ± 3.54 (P < 0.001), and the RV myocardial performance increased from 0.20 ± 0.05 to 0.63 ± 0.16 (P < 0.001). On CT, the RV end-systolic volume increased from 11.11 ± 1.81 ml to 24.71 ± 4.60 ml (P < 0.001), RV end-diastolic volume from 20.73 ± 2.83 ml to 34.63 ± 5.76 ml (P < 0.001), and the four-chamber RV/left ventricular diameter ratio from 0.38 ± 0.07 to 0.81 ± 0.14 (P < 0.001).</p> </sec> <sec> <title id="st0025">Conclusion</title> <p id="sp0020">Acute PE with RV dysfunction was established in a large animal model through controlled injection of autologous blood clots, which may be useful for developing and evaluating new therapeutic approaches for acute PE with RV dysfunction.</p> </sec> </abstract>