Adjunctive biomarkers for improving diagnosis of tuberculosis and monitoring therapeutic effects. Issue 4 (April 2015)

• Record Type: Journal Article
• Authors: Hur, Yun-Gyoung; Kang, Young Ae; Jang, Sun-Hee; Hong, Ji Young; Kim, Ahreum; Lee, Sang A; Kim, Youngmi; Cho, Sang-Nae
• Is Part Of: Journal of infection. Volume 70:Issue 4(2015)
• Page Start: 346
• Page End: 355
• Publication Date: 2015-04
• Subjects: Infection -- Periodicals; Bacterial Infections -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.905
• DOI: 10.1016/j.jinf.2014.10.019
• Languages: English
• ISSNs: 0163-4453

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Summary</title> <sec> <title id="sectitle0015">Objectives</title> <p id="abspara0010">To identify host biomarkers associated with latent tuberculosis infection (LTBI), active tuberculosis (TB), and nontuberculous mycobacteria (NTM) diseases to improve diagnosis and effective anti-TB treatment.</p> </sec> <sec> <title id="sectitle0020">Methods</title> <p id="abspara0015">Active TB and NTM patients at diagnosis, recent TB contacts, and normal healthy subjects were recruited. Tuberculin skin tests, QuantiFERON-TB Gold In-Tube tests, and multiplex bead arrays with 17 analytes were performed. TB patients were re-evaluated after 2 and 6 months of treatment.</p> </sec> <sec> <title id="sectitle0025">Results</title> <p id="abspara0020"> <italic>Mycobacterium tuberculosis</italic> (<italic>M. tb</italic>) antigen-specific IFN-γ, IL-2, and CXCL10 responses were significantly higher in active TB and LTBI compared with controls (<italic>P</italic> < 0.01). Only serum VEGF levels varied between the active TB and LTBI groups (AUC = 0.7576, <italic>P</italic> < 0.001). Active TB and NTM diseases were differentiated by serum IL-2, IL-9, IL-13, IL-17, TNF-α and sCD40L levels (<italic>P</italic> < 0.05). Increased sCD40L and decreased <italic>M. tb</italic> antigen-specific IFN-γ levels correlated with sputum clearance of <italic>M. tb</italic> after 2 months of treatment (<italic>P</italic> < 0.001).</p> </sec> <sec> <title id="sectitle0030">Conclusions</title> <p id="abspara0025">Serum IL-2, IL-9, IL-13, IL-17, TNF-α, sCD40L and VEGF-A levels may be adjunctive biomarkers for differential diagnosis of active TB, LTBI, and NTM disease. Assessment of serum sCD40L and <italic>M. tb</italic> antigen-specific IFN-γ, TNF-α, and IL-2 levels could help predict successful anti-TB treatment in conjunction with <italic>M. tb</italic> clearance.</p> </sec> </abstract>