Intestinal absorption and cell transforming potential of PhIP-M1, a bacterial metabolite of the heterocyclic aromatic amine 2-amino-1-methyl-6-phenylimidazo[4, 5-b]pyridine (PhIP). Issue 2 (16th April 2015)

• Record Type: Journal Article
• Authors: Nicken, Petra; Willenberg, Ina; Keutz, Anne von; Elsner, Leonie von; Hamscher, Gerd; Vanhaecke, Lynn; Schröder, Bernd; Breves, Gerhard; Schebb, Nils Helge; Steinberg, Pablo
• Is Part Of: Toxicology letters. Volume 234:Issue 2(2015)
• Page Start: 92
• Page End: 98
• Publication Date: 2015-04-16
• Subjects: Toxicology -- Periodicals; 363.179
• DOI: 10.1016/j.toxlet.2015.02.015
• Languages: English
• ISSNs: 0378-4274

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0020"> <list id="lis0005"> <list-item id="lsti0005"> <label></label> <p id="par0005">The transport of PhIP-M1, a metabolite of the colon carcinogen PhIP produced by gut bacteria, in eight different intestinal segments from male Fischer 344 rats was examined in Ussing chambers.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">A very recently developed HPLC method to quantify the concentration of PhIP-M1 in biological fluids as well as in intestinal tissue was applied.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">At the most, 10–20% of the PhIP-M1 amount added to the mucosal compartment of the Ussing chambers per segment were absorbed within 90 min.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">PhIP-M1 in a concentration of up to 100 μM did not induce the malignant transformation of BALB/c 3T3 cells.</p> </list-item> <list-item id="lsti0025"> <label></label> <p id="par0025">Even if one would assume that 100% of the daily amount of PhIP ingested by a human being is converted into PhIP-M1 in the colon, this concentration most probably would not lead to carcinogenicity in the colorectal mucosa.</p> </list-item> </list> </p> </sec> </abstract>