Isolation and characterization of biologically active alkaloids from marine actinobacteria Nocardiopsis sp. NCS1. Issue 1 (January 2015)

• Record Type: Journal Article
• Authors: Kamala, K.; Sivaperumal, P.; Gobalakrishnan, R.; Swarnakumar, N.S.; Rajaram, R.
• Is Part Of: Biocatalysis and agricultural biotechnology. Volume 4:Issue 1(2015)
• Page Start: 63
• Page End: 69
• Publication Date: 2015-01
• Subjects: Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2014.10.005
• Languages: English
• ISSNs: 1878-8181

Abstract

<abstract abstract-type="author" id="ab0005"> <title id="sect0005">Abstract</title> <sec> <p id="sp0080">Actinobacteria have special biotechnological importance since they produce chemically diverse compounds with a sample range of biological activity. In the present study the potential alkaloids producing marine actinobacterial strain NCS1 was isolated from the sediments of Nancowry Island, India which was identified as <italic>Nocardiopsis</italic> sp. based on chemotaxonomy, cultural, morphological and 16S rDNA sequencing. Further, the crude alkaloids were extracted and purified through column chromatography and characterized using a UV spectrophotometer and FT-IR spectrometer. It has potential antioxidant efficacy and cytotoxic effect against NCI-H460 cells. The highest antioxidant activity was observed at 100 µg/ml concentration for instance DPPH assay, total antioxidant, total reducing power, hydrogen peroxide, nitrous oxide and lipid peroxidation, respectively. Moreover, the cytotoxicity assay was done against NCI-H460 cell lines and IC₅₀ value was found to be 40 μg/ml. These results suggest that <italic>Nocardiopsis</italic> sp. NCS1 has novel antioxidant, cytotoxic activity and it has to be further characterized to improve the pharmacological native marine metabolites.</p> </sec> </abstract>