Early steps in the European eel (Anguilla anguilla)–Vibrio vulnificus interaction in the gills: Role of the RtxA13 toxin. Issue 2 (April 2015)

• Record Type: Journal Article
• Authors: Callol, Agnès; Pajuelo, David; Ebbesson, Lars; Teles, Mariana; MacKenzie, Simon; Amaro, Carmen
• Is Part Of: Fish & shellfish immunology. Volume 43:Issue 2(2015:Apr.)
• Page Start: 502
• Page End: 509
• Publication Date: 2015-04
• Subjects: Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2015.01.009
• Languages: English
• ISSNs: 1050-4648

Abstract

<abstract xml:lang="en" abstract-type="author" id="abs0010"> <title id="sectitle0010">Abstract</title> <sec> <p id="abspara0010"> <italic>Vibrio vulnificus</italic> is an aquatic gram-negative bacterium that causes a systemic disease in eels called warm-water vibriosis. Natural disease occurs via water born infection; bacteria attach to the gills (the main portal of entry) and spread to the internal organs through the bloodstream, provoking host death by haemorrhagic septicaemia. <italic>V. vulnificus</italic> produces a toxin called RtxA1<sub>3</sub> that hypothetically interferes with the eel immune system facilitating bacterial invasion and subsequent death by septic shock. The aim of this work was to study the early steps of warm-water vibriosis by analysing the expression of three marker mRNA transcripts related to pathogen recognition (<italic>tlr2</italic> and <italic>tlr5</italic>) and inflammation (<italic>il-8</italic>) in the gills of eels infected by immersion with either the pathogen or a mutant deficient in <italic>rtxA1</italic><sub><italic>3</italic></sub>. Results indicate a differential response that is linked to the <italic>rtx</italic> toxin in the expression levels of the three measured mRNA transcripts. The results suggest that eels are able to distinguish innocuous from harmful microorganisms by the local action of their toxins rather than by surface antigens. Finally, the cells that express these transcripts in the gills are migratory cells primarily located in the second lamellae that re-locate during infection suggesting the activation of a specific immune response to pathogen invasion in the gill.</p> </sec> </abstract>