Tuberculosis drug resistance isolates from pulmonary tuberculosis patients, Kassala State, Sudan. Issue 1 (March 2015)

• Record Type: Journal Article
• Authors: Khalid, Fatima A.; Hamid, Zuhal A.; Mukhtar, M.M.
• Is Part Of: International journal of mycobacteriology. Volume 4:Issue 1(2015:Mar.)
• Page Start: 44
• Page End: 47
• Publication Date: 2015-03
• Subjects: Mycobacteria -- Periodicals; Mycobacterial diseases -- Periodicals; Mycobacteriaceae; Mycobacteria; Electronic journals; Periodicals; 579.374
• DOI: 10.1016/j.ijmyco.2014.11.064
• Languages: English
• ISSNs: 2212-5531

Abstract

<abstract xml:lang="en" abstract-type="author" id="ab005"> <title id="st090">Abstract</title> <sec> <title id="st050">Background</title> <p id="sp0005">This study was conducted in Kassala Teaching Hospital, Kassala State, Sudan (January 2006–June 2008) to determine the rate of mycobacterium drug resistance to anti-tuberculous treatment and to explore the genotype of <italic>Mycobacterium tuberculosis</italic> resistant isolates using <italic>rpoB</italic> gene.</p> </sec> <sec> <title id="st055">Methods</title> <p id="sp0010">53 isolates of mycobacterium isolated from pulmonary tuberculosis (PTB) patients from Kassala State were subjected to drug susceptibility testing (DST) to anti-tuberculous drugs; 10 <italic>M.</italic><italic>tuberculosis</italic> complex (MTBC) resistant isolates were subjected to polymerase chain reaction (PCR), and commercially the amplified DNA was sequenced.</p> </sec> <sec> <title id="st060">Results</title> <p id="sp0015">DST detected resistance in 23/53 (43.39%) isolates, among which rifampicin had a high number of resistant isolates (13/23), followed by streptomycin (11/23), and multi-drug resistance was detected in 5 isolates.</p> <p id="sp0020">DNA sequence analysis of 10 MTBC-resistant isolates detected variations within and outside the rifampicin resistant determining region (RRDR). Variation within RRDR was detected at positions 512 (AGC/ATC, Ser/Ile), and 528 (CGC/CTC, Arg/Leu). Outside the RRDR region variations were detected at positions 498 (GTG/GGG, Val/gly), 488 (ACA/ACC, Thr/Thr), which is a silent mutation. Insertions were observed at positions 484, 496 (GTG/GTGA, CGG/CAGG, respectively). Deletion was observed at position 487 (ATC/_TC).</p> </sec> <sec> <title id="st065">Discussion and conclusion</title> <p id="sp0025">This study revealed that high resistance to rifampicin was associated with various point mutations in and out of the RRDR of the <italic>rpoB</italic> gene. Molecular methods are needed for early detection of TB disease and drug resistance.</p> </sec> </abstract>