Production and immunological analysis of IgE reactive recombinant egg white allergens expressed in Escherichia coli. Issue 1 (May 2015)

• Record Type: Journal Article
• Authors: Dhanapala, Pathum; Doran, Tim; Tang, Mimi L.K.; Suphioglu, Cenk
• Is Part Of: Molecular immunology. Volume 65:Issue 1(2015:May)
• Page Start: 104
• Page End: 112
• Publication Date: 2015-05
• Subjects: Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2015.01.006
• Languages: English
• ISSNs: 0161-5890

Abstract

<abstract abstract-type="author-highlights" id="abs0005"> <title id="sect0005">Highlights</title> <sec> <p id="spar0005"> <list id="lis0005"> <list-item id="lsti0005"> <label></label> <p id="par0005">We successfully produced recombinant versions of Gal d 1, 2 and 3, that were IgE reactive when tested against a pool of egg allergic patients' sera.</p> </list-item> <list-item id="lsti0010"> <label></label> <p id="par0010">The IgE reactivity indicates that these recombinant proteins are capable of eliciting an immune response, thus being potential candidates for immunotherapy.</p> </list-item> <list-item id="lsti0015"> <label></label> <p id="par0015">We have, for the first time, attempted to produce recombinant versions of all 4 major egg white allergens in <italic>E. coli</italic>, and successfully produced 3, with only Gal d 4 showing loss of IgE reactivity in the recombinant version.</p> </list-item> <list-item id="lsti0020"> <label></label> <p id="par0020">This is the first report of a collective and comparative immunological analysis of all 4 egg white allergens.</p> </list-item> <list-item id="lsti0025"> <label></label> <p id="par0025">The significance of this study is the potential use of the IgE reactive recombinant egg white proteins in immunotherapy to treat egg allergic patients.</p> </list-item> </list> </p> </sec> </abstract>