Characterization of the in vivo host response to a bi‐labeled chitosan‐dextran based hydrogel for postsurgical adhesion prevention. Issue 8 (31st December 2014)

• Record Type: Journal Article
• Authors: Cabral, Jaydee D.; McConnell, Michelle A.; Fitzpatrick, Clare; Mros, Sonya; Williams, Gail; Wormald, Peter J.; Moratti, Stephen C.; Hanton, Lyall R
• Is Part Of: Journal of biomedical materials research. Volume 103:Issue 8(2015:Aug.)
• Page Start: 2611
• Page End: 2620
• Publication Date: 2014-12-31
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.a.35395
• Languages: English
• ISSNs: 1549-3296

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>In developing a chitosan/dextran‐based (CD) hydrogel as an adhesion prevention postsurgical aid, the <italic>in vivo</italic> biodegradation rate, biodistribution, and inflammatory response are important parameters to the biomedical device design. Herein, for the first time, a CD hydrogel was prepared by mixing aqueous solutions of a near infrared (NIR) labeled succinylated chitosan (SC) and tritiated [<sup>3</sup>H] oxidized dextran (DA). The biodegradation and biodistribution of the NIR/[<sup>3</sup>H]‐CD hydrogel was tracked noninvasively using NIR fluorescence imaging, and by liquid scintillation counting (LSC) of organs/tissues after subcutaneous injection in BALB/c mice. The inflammatory response was assessed by measuring serum cytokine levels using a Bio‐plex assay and by histological examination of injection site tissue. Fluorescence imaging showed the hydrogel to degrade in under a week. LSC revealed the hydrogel to reside mainly at the injection site, and excreted primarily via the urine within the first 48 h. The CD hydrogel showed a mild inflammatory response as cytokine levels were comparable to saline injected controls. Histological examination of injection site tissue confirmed the cytokine results. In summary, the CD hydrogel's <italic>in vivo</italic> biodegradation rate, biodistribution, and inflammatory response was determined. Our results indicate that the CD hydrogel has an appropriate biocompatibility after s.c. administration. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2611–2620, 2015.</p> </abstract>