Stem bark and flower extracts of Vismia cauliflora are highly effective antioxidants to human blood cells by preventing oxidative burst in neutrophils and oxidative damage in erythrocytes. (November 2015)

• Record Type: Journal Article
• Authors: Ribeiro, Alessandra Braga; Berto, Alessandra; Ribeiro, Daniela; Freitas, Marisa; Chisté, Renan Campos; Visentainer, Jesuí Vergílio; Fernandes, Eduarda
• Is Part Of: Pharmaceutical biology. Volume 53:Number 11(2015:Nov.)
• Page Start: 1691
• Page End: 1698
• Publication Date: 2015-11
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2014.1001407
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: <italic>Vismia cauliflora</italic> A.C.Sm<italic>.</italic> [Hypericaceae (Clusiaceae)] is an Amazonian plant traditionally used by indigenous population to treat dermatosis and inflammatory processes of the skin. Previous research on <italic>V. cauliflora</italic> extracts suggests its potential to neutralize cellular oxidative damages related to the production of reactive oxygen and nitrogen species.</p> <p> <italic>Objective</italic>: To determine the activity of stem bark and flower extracts of <italic>V. cauliflora</italic> on the modulation of oxidative burst in human neutrophils, as well as its potential to inhibit oxidative damage in human erythrocytes.</p> <p> <italic>Materials and methods</italic>: The modulation of neutrophil's oxidative burst by the ethanolic extracts (0.3–1000 µg/mL) was determined by the oxidation of specific probes by reactive species. Additionally, the potential of these extracts to inhibit oxidative damage in human erythrocytes was evaluated by monitoring its biomarkers of oxidative stress.</p> <p> <italic>Results</italic>: <italic>Vismia cauliflora</italic> extracts presented remarkable capacity to prevent the oxidative burst in activated human neutrophils (IC<sub>50</sub> < 15 µg/mL). However, the maximum percentage of inhibition achieved against hydrogen peroxide was 45%. Concerning the oxidative damage in human erythrocytes, the extracts were able to minimize the <italic>tert</italic>-butyl hydroperoxide-induced hemoglobin oxidation and lipid peroxidation in a very low concentration range (2.7–18 μg/mL). Furthermore, only stem bark extract (100 µg/mL) was able to inhibit the depletion of glutathione (13%).</p> <p> <italic>Discussion and conclusion</italic>: These results reinforce the therapeutic potential of stem bark and flower extracts of <italic>V. cauliflora</italic> to heal topical skin disease, namely in the treatment of neutrophil-related dermatosis and skin conditions related to oxidative stress, including skin aging.</p> </abstract>