Attenuation of hyperoxia-induced lung injury in neonatal rats by 1α, 25-Dihydroxyvitamin D3. (1st August 2015)

• Record Type: Journal Article
• Authors: Chen, Yan; Li, Qiong; Liu, Yalan; Shu, Li; Wang, Na; Wu, Yipin; Sun, Xue; Wang, Lin
• Is Part Of: Experimental lung research. Volume 41:Number 6(2015:Jun.)
• Page Start: 344
• Page End: 352
• Publication Date: 2015-08-01
• Subjects: Lungs -- Periodicals; Lungs -- Diseases -- Periodicals; Lung Diseases; Lung -- physiology; Respiratory System; 616.24
• DOI: 10.3109/01902148.2015.1039668
• Languages: English
• ISSNs: 0190-2148

Abstract

<abstract> <title>ABSTRACT</title> <p> <italic>Background</italic>: Mounting evidence suggests that Toll-like receptor (TLRs) plays an important role in oxidative stress and is implicated in the pathogenesis of hyperoxic lung injury. 1α, 25-Dihydroxyvitamin D<sub>3</sub> (1, 25(OH)<sub>2</sub>D<sub>3</sub>), the hormonally active form of vitamin D, not only plays an essential role in mineral balance, but also possesses immunomodulatory and antioxidant properties. Besides, Vitamin D<sub>3</sub> is involved in the regulation of TLRs signaling. The present study was designed to investigate whether 1, 25(OH)<sub>2</sub>D<sub>3</sub> attenuates hyperoxia-induced lung injury by regulating TLRs signaling in neonatal rats. <italic>Methods</italic>: Pups were divided into four groups: normoxia control group (NC), normoxia plus 1, 25(OH)<sub>2</sub>D<sub>3</sub> treatment group (ND), hyperoxia control group (HC), and hyperoxia plus 1, 25(OH)<sub>2</sub>D<sub>3</sub> treatment group (HD). Lung tissues were collected for histological examination and detection of mRNA and protein expressions. <italic>Results</italic>: Treatment of hyperoxia-exposed animals with 1, 25(OH)<sub>2</sub>D<sub>3</sub> resulted in significantly increased body weight and reduced hyperoxia-induced lung injury. Moreover, 1, 25(OH)<sub>2</sub>D<sub>3</sub> significantly downregulated the expression of TLR4, NF-κB, and the inflammatory cytokines TNF-α, IL-1β, and IL-6. <italic>Conclusions</italic>: 1, 25(OH)<sub>2</sub>D<sub>3</sub> could attenuate hyperoxia-induced lung injury in neonatal rats, possibly by regulating TLR4/NF-κB signaling.</p> </abstract>