Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells. Issue 8 (August 2015)

• Record Type: Journal Article
• Authors: Scheffel, Débora Lopes Salles; Soares, Diana Gabriela; Basso, Fernanda Gonçalves; de Souza Costa, Carlos Alberto; Pashley, David; Hebling, Josimeri
• Is Part Of: Journal of dentistry. Volume 43:Issue 8(2015:Aug.)
• Page Start: 997
• Page End: 1006
• Publication Date: 2015-08
• Subjects: Dentistry -- Periodicals; Dentistry -- Periodicals; Dentisterie -- Périodiques; Electronic journals; 617.6005
• DOI: 10.1016/j.jdent.2015.05.004
• Languages: English
• ISSNs: 0300-5712

Abstract

<abstract abstract-type="author" id="abs0005"> <title id="sect0005">Abstract</title> <sec> <title id="sect0010">Objectives</title> <p id="spar0005">This study investigated the transdentinal cytotoxicity of glutahaldehyde-containing solutions/materials on odontoblast-like cells.</p> </sec> <sec> <title id="sect0015">Methods</title> <p id="spar0010">Dentin discs were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal side of the discs and the occlusal surface was treated with the following solutions: water, 2% glutaraldehyde (GA), 5% GA, 10% GA, Gluma Comfort Bond + Desensitizer (GCB + De) or Gluma Desensitizer (GDe). Cell viability and morphology were assessed by the Alamar Blue assay and SEM. The eluates were collected and applied on cells seeded in 24-well plates. After 7 or 14 days the total protein (TP) production, alkaline phosphatase activity (ALP) and deposition of mineralized nodules (MN) were evaluated.</p> </sec> <sec> <title id="sect0020">Results</title> <p id="spar0015">Data were analyzed by Kruskal-Wallis and Mann-Whitney tests (<italic>p</italic> < 0.05). GA solutions were not cytotoxic against MDPC-23. GCB + De (85.1%) and GDe (77.2%) reduced cell viability as well as TP production and ALP activity at both periods. After 14 days, GCB + De and GDe groups produced less MN. Affected MDPC-23 presented deformation of the cytoskeleton and reduction of cellular projections.</p> </sec> <sec> <title id="sect0025">Conclusions</title> <p id="spar0020">The treatment with 2.5%, 5% and 10% GA was not harmful to odontoblast-like cells. Conversely, when GA was combined with other components like HEMA, the final material became cytotoxic.</p> </sec> <sec> <title id="sect0030">Clinical significance</title> <p id="spar0025">Glutaraldehyde has been used to decrease dentin hypersensitivity. This substance is also capable of preventing resin-dentin bond degradation by cross-linking collagen and MMPs. This study showed that GA might be safe when applied on acid etched dentin. However, when combined with HEMA the product becomes cytotoxic.</p> </sec> </abstract>