IGF1 Promotes Adipogenesis by a Lineage Bias of Endogenous Adipose Stem/Progenitor Cells. (27th May 2015)

• Record Type: Journal Article
• Authors: Hu, Li; Yang, Guodong; Hägg, Daniel; Sun, Guoming; Ahn, Jeffrey M.; Jiang, Nan; Ricupero, Christopher L.; Wu, June; Rodhe, Christine Hsu; Ascherman, Jeffrey A.; Chen, Lili; Mao, Jeremy J.
• Is Part Of: Stem cells. Volume 33:Number 8(2015:Aug.)
• Page Start: 2483
• Page End: 2495
• Publication Date: 2015-05-27
• Subjects: Cloning -- Periodicals; Clone cells -- Periodicals; Stem cells -- Periodicals; Cell Differentiation -- Periodicals; Cell Division -- Periodicals; Clone Cells -- Periodicals; Hematopoietic Stem Cells -- Periodicals; Stem Cells -- Periodicals; 571.84
• DOI: 10.1002/stem.2052
• Languages: English
• ISSNs: 1066-5099

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>Adipogenesis is essential for soft tissue reconstruction following trauma or tumor resection. We demonstrate that CD31<sup>−</sup>/34<sup>+</sup>/146<sup>−</sup> cells, a subpopulation of the stromal vascular fraction (SVF) of human adipose tissue, were robustly adipogenic. <italic>Insulin growth factor‐1 (IGF1)</italic> promoted a lineage bias towards CD31<sup>−</sup>/34<sup>+</sup>/146<sup>−</sup> cells at the expense of CD31<sup>−</sup>/34<sup>+</sup>/146<sup>+</sup> cells. <italic>IGF1</italic> was <italic>microencapsulated</italic> in poly(lactic‐<italic>co</italic>‐glycolic acid) scaffolds and implanted in the inguinal fat pad of C57Bl6 mice. Control‐released <italic>IGF1</italic> induced remarkable adipogenesis in vivo by recruiting endogenous cells. In comparison with the CD31<sup>−</sup>/34<sup>+</sup>/146<sup>+</sup> cells, CD31<sup>−</sup>/34<sup>+</sup>/146<sup>−</sup> cells had a weaker <italic>Wnt/β‐catenin</italic> signal. IGF1 attenuated <italic>Wnt/β‐catenin</italic> signaling by activating <italic>Axin2/PPARγ</italic> pathways in SVF cells, suggesting <italic>IGF1</italic> promotes CD31<sup>−</sup>/34<sup>+</sup>/146<sup>−</sup> bias through tuning <italic>Wnt</italic> signal. <italic>PPARγ</italic> response element (PPRE) in <italic>Axin2</italic> promoter was crucial for <italic>Axin2</italic> upregulation, suggesting that <italic>PPARγ</italic> transcriptionally activates <italic>Axin2</italic>. Together, these findings illustrate an <italic>Axin2/PPARγ</italic> axis in adipogenesis that is particularly attributable to a lineage bias towards CD31<sup>−</sup>/34<sup>+</sup>/146<sup>−</sup> cells, with implications in adipose regeneration. S<sc>tem</sc> C<sc>ells</sc><italic>2015;33:2483–2495</italic></p> </abstract>