Detection and molecular characterisation of Giardia duodenalis, Cryptosporidium spp. and Entamoeba spp. among patients with gastrointestinal symptoms in Gambo Hospital, Oromia Region, southern Ethiopia. Issue 9 (19th May 2015)

• Record Type: Journal Article
• Authors: Flecha, María J.; Benavides, Cynthia M.; Tissiano, Gabriel; Tesfamariam, Abraham; Cuadros, Juan; de Lucio, Aida; Bailo, Begoña; Cano, Lourdes; Fuentes, Isabel; Carmena, David
• Is Part Of: Tropical medicine & international health. Volume 20:Issue 9(2015:Sep.)
• Page Start: 1213
• Page End: 1222
• Publication Date: 2015-05-19
• Subjects: Tropical medicine -- Periodicals; Public health -- Periodicals; 616.988
• DOI: 10.1111/tmi.12535
• Languages: English
• ISSNs: 1360-2276

Abstract

<abstract abstract-type="main" id="tmi12535-abs-0001"> <title>Summary</title> <sec id="tmi12535-sec-0001" sec-type="section"> <title>Objectives</title> <p>To assess the prevalence and genetic diversity of the enteric protozoa species <italic>G. duodenalis</italic>, <italic> Cryptosporidium</italic> spp. and <italic>Entamoeba histolytica</italic> in individuals with gastrointestinal symptoms compatible with infections by these pathogens seeking medical attention in a rural area in southern Ethiopia.</p> </sec> <sec id="tmi12535-sec-0002" sec-type="section"> <title>Methods</title> <p>A total of 92 stool samples were initially screened by direct microscopy and immunochromatography and further confirmed by molecular methods. <italic>G. duodenalis</italic>‐positive samples were molecularly characterised by multilocus genotyping of the glutamate dehydrogenase and <italic>β</italic>‐giardin genes of the parasite. PCR and DNA sequence analysis of the gene encoding the 60‐kDa glycoprotein was used for the subtyping of <italic>Cryptosporidium</italic> isolates. Detection and differential diagnosis of <italic>E. histolytica</italic>/<italic>dispar</italic> were conducted by real‐time PCR.</p> </sec> <sec id="tmi12535-sec-0003" sec-type="section"> <title>Results</title> <p>PCR‐based prevalences were 10.9% for <italic>G. duodenalis</italic>, 1.1% for <italic>Cryptosporidium</italic> spp. and 3.3% for <italic>Entamoeba</italic> spp. Seven (four novel and three known) subtypes of <italic>G. duodenalis</italic> assemblage B were identified at the <italic>GDH</italic> locus and 5 (one novel and four known) at the <italic>BG</italic> locus. A novel variant of <italic>C. hominis</italic> subtype IbA9G3 was also identified. Two <italic>Entamoeba</italic> isolates were assigned to <italic>E. dispar</italic> and an additional one to <italic>E. histolytica</italic>.</p> </sec> <sec id="tmi12535-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Although preliminary, our results strongly suggest that giardiasis, cryptosporidiosis and amoebiasis represent a significant burden in Ethiopian rural population.</p> </sec> </abstract>