Surface‐Tunable Bioluminescence Resonance Energy Transfer via Geometry‐Controlled ZnO Nanorod Coordination. Issue 28 (19th March 2015)

• Record Type: Journal Article
• Authors: Lim, Jun Hyung; Park, Geun Chul; Lee, Seung Muk; Lee, Jung Heon; Lim, Butaek; Hwang, Soo Min; Kim, Jung Ho; Park, Hansoo; Joo, Jinho; Kim, Young‐Pil
• Is Part Of: Small. Volume 11:Issue 28(2015)
• Page Start: 3469
• Page End: 3475
• Publication Date: 2015-03-19
• Subjects: Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201403700
• Languages: English
• ISSNs: 1613-6810

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The use of ZnO nanorods (NRs) as an effective coordinator and biosensing platform to create bioluminescence resonance energy transfer (BRET) is reported. Herein, a hydrothermal approach is applied to obtain morphologically controlled ZnO NRs, which are directly bound to luciferase (Luc) and carboxy‐modified quantum dot (QD) acting as a donor–acceptor pair for BRET. BRET efficiency varies significantly with the geometry of ZnO NRs, which modulates the coordination between hexahistidine‐tagged Luc (Luc‐His₆) and QD, owing to the combined effect of the total surface area consisting of (001) and (100) planes and their surface polarities. Unlike typical QD–BRET reactions with metal ions (e.g., zinc ions), a geometry‐controlled ZnO NR platform can facilitate the design of surface‐initiated BRET sensors without being supplemented by copious metal ions: the geometry‐controlled ZnO NR platform can therefore pave the way for nanostructure‐based biosensors with enhanced analytical performance.</p> </abstract>