Capsaicin reduces the metastatic burden in the transgenic adenocarcinoma of the mouse prostate model. Issue 12 (5th June 2015)

• Record Type: Journal Article
• Authors: Venier, Natalie A.; Yamamoto, Toshihiro; Sugar, Linda M.; Adomat, Hans.; Fleshner, Neil E.; Klotz, Laurence H.; Venkateswaran, Vasundara
• Is Part Of: Prostate. Volume 75:Issue 12(2015)
• Page Start: 1300
• Page End: 1311
• Publication Date: 2015-06-05
• Subjects: Prostate -- Diseases -- Periodicals; 616
• DOI: 10.1002/pros.23013
• Languages: English
• ISSNs: 0270-4137

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="pros23013-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p>Capsaicin, the active compound in chili peppers, has demonstrated anti‐ carcinogenic properties <italic>in vitro</italic> in a number of malignancies, including the prostate. In the present study, we investigate the chemopreventive potential of capsaicin on prostate cancer using the transgenic adenocarcinoma of the mouse prostate (TRAMP) model. The TRAMP is a murine model that resembles the progression of human disease.</p> </sec> <sec id="pros23013-sec-0002" sec-type="section"> <title>METHODS</title> <p>Thirty‐five 6‐week‐old TRAMP x C57BL/6 mice were randomized between treatment with capsaicin (5 mg/kg body weight) or control (saline) three times a week by oral gavage until 30 weeks of age. Body weight of animals was recorded thrice weekly. At termination, all tumors were extracted, recorded, and analyzed for histopathological analysis. To understand the effect of capsaicin on migration and invasion, <italic>in vitro</italic> experiments were carried out using PC3 cells.</p> </sec> <sec id="pros23013-sec-0003" sec-type="section"> <title>RESULTS</title> <p>Mice in the control group expressed an overall trend of higher‐grade disease with 37.5% poorly differentiated (PD), 18.75% moderately differentiated (MD), and 44% of well‐differentiated (WD) adenocarcinoma, compared to the capsaicin‐treated group with only 27.7% PD, 61.0% of WD, and 11.1% of intraepithelial neoplasia (PIN). The treatment group demonstrated a higher incidence of noncancerous PIN lesions compared to the control group. The capsaicin group also demonstrated a significant reduction (<italic>P</italic> < 0.05) in the metastatic burden compared to the controls, which correlated to a reduction in p27<italic><sup>Kip</sup><sup>1</sup></italic> expression and neuroendocrine differentiation in prostate tumors. Furthermore, there were no differences in body weight between groups overtime, and no pathological toxicities in the liver and gastrointestinal tract with capsaicin consumption. In vitro studies revealed a dose‐dependent reduction in the invasion and migration capacity of PC3 cells.</p> </sec> <sec id="pros23013-sec-0004" sec-type="section"> <title>CONCLUSION</title> <p>The following study provides evidence supporting the safety and chemopreventive effects of capsaicin in the TRAMP model. <italic>Prostate 75:1300–1311, 2015</italic>. © 2015 Wiley Periodicals, Inc.</p> </sec> </abstract>