Staphylococcal enterotoxin B induces specific IgG4 and IgE antibody serum levels in atopic dermatitis. (14th October 2014)

• Record Type: Journal Article
• Authors: Orfali, Raquel L.; Sato, Maria N.; Santos, Vanessa G.; Titz, Tiago O.; Brito, Cyro A.; Duarte, Alberto J. S.; Takaoka, Roberto; Aoki, Valéria
• Is Part Of: International journal of dermatology. Volume 54:Number 8(2015:Aug.)
• Page Start: 898
• Page End: 904
• Publication Date: 2014-10-14
• Subjects: Dermatology -- Periodicals; 616.5
• DOI: 10.1111/ijd.12533
• Languages: English
• ISSNs: 0011-9059

Abstract

<abstract abstract-type="main" id="ijd12533-abs-0001"> <title>Abstract</title> <sec id="ijd12533-sec-0001" sec-type="section"> <title>Background</title> <p>Atopic dermatitis (AD) is a chronic recurrent inflammatory disease, with prevalence of about 10–20% in children and 1–3% in adults. <italic>Staphylococcus aureus</italic> is present in 80–100% of skin from atopic patients and is related to worsening of the disease by the action of enterotoxins. The aim of this study was to evaluate the profile of anti‐<italic>Staphylococcus aureus</italic> enterotoxin B (SEB) antibody isotypes and IgG subclass levels in adult AD.</p> </sec> <sec id="ijd12533-sec-0002" sec-type="section"> <title>Methods</title> <p>We selected 38 patients with AD, diagnosed by Hanifin and Rajka's criteria, aged between 18 and 65, and 26 healthy controls (HC). The severity of the disease was established according to the Eczema Area and Severity Index and patients graded as mild (28%), moderate (58%), and severe (14%). Sera were assessed for IgG subclasses, IgA, IgM, and IgE against SEB by ELISA.</p> </sec> <sec id="ijd12533-sec-0003" sec-type="section"> <title>Results</title> <p>Elevated circulating IgE and IgG4 anti‐SEB antibody levels associated with decreased IgA and IgM levels were detected in patients with AD, when compared to HC individuals. The severity of AD was related to low IgG1 and IgG3 levels and a high IgE antibody response to SEB. Interestingly, absence of IgG4 response to SEB was lower in patients with AD (2.63%), when compared to controls (34.6%), while a similar absence was detected for IgG1 and IgE antibodies (AD, 23.3 and 18.4% vs. HC, 38.5 and 19.2%).</p> </sec> <sec id="ijd12533-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Our findings evidenced a contributing role for IgG4 and IgE antibodies in AD pathogenesis, which are triggered by staphylococcal superantigens.</p> </sec> </abstract>