Characterization of the cultivable bacterial populations associated with field grown Brassica napus L.: an evaluation of sampling and isolation protocols. (27th January 2015)

• Record Type: Journal Article
• Authors: Croes, Sarah; Weyens, Nele; Colpaert, Jan; Vangronsveld, Jaco
• Is Part Of: Environmental microbiology. Volume 17:Number 7(2015:Jul.)
• Page Start: 2379
• Page End: 2392
• Publication Date: 2015-01-27
• Subjects: Microbial ecology -- Periodicals; Environmental Microbiology -- Periodicals; 579.17
• DOI: 10.1111/1462-2920.12701
• Languages: English
• ISSNs: 1462-2912

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>Plant‐associated bacteria are intensively investigated concerning their characteristics for plant growth promotion, biocontrol mechanisms and enhanced phytoremediation efficiency. To obtain endophytes, different sampling and isolation protocols are used although their representativeness is not always clearly demonstrated. The objective of this study was to acquire representative pictures of the cultivable bacterial root, stem and leaf communities for all <italic>B</italic><italic>rassica napus</italic> L. individuals growing on the same field.</p> <p>For each plant organ, genotypic identifications of the endophytic communities were performed using three replicates. Root replicates were composed of three total root systems, whereas stem and leaf replicates needed to consist of six independent plant parts in order to be representative. Greater variations between replicates were found when considering phenotypic characteristics. Correspondence analysis revealed reliable phenotypic results for roots and even shoots, but less reliable ones for leaves. Additionally, realistic Shannon–Wiener biodiversity indices were calculated for all three organs and showed similar Evenness factors.</p> <p>Furthermore, it was striking that all replicates and thus the whole plant contained <italic>P</italic><italic>seudomonas</italic> and <italic>B</italic><italic>acillus</italic> strains although aboveground and belowground plant tissues differed in most dominant bacterial genera and characteristics.</p> </abstract>