Uncultured Desulfobacteraceae and Crenarchaeotal group C3 incorporate 13C‐acetate in coastal marine sediment. Issue 4 (18th June 2015)

• Record Type: Journal Article
• Authors: Na, Hyunsoo; Lever, Mark Alexander; Kjeldsen, Kasper Urup; Schulz, Frederik; Jørgensen, Bo Barker
• Is Part Of: Environmental microbiology reports. Volume 7:Issue 4(2015)
• Page Start: 614
• Page End: 622
• Publication Date: 2015-06-18
• Subjects: Microbial ecology -- Periodicals; Environmental Microbiology -- Periodicals; Electronic journals; 579.17
• DOI: 10.1111/1758-2229.12296
• Languages: English
• ISSNs: 1758-2229

Abstract

<abstract abstract-type="main"> <title>Summary</title> <p>Stable isotope probing (SIP) of deoxyribonucleic acid (DNA) was used to identify microbes incorporating <sup>13</sup>C‐labeled acetate in sulfate‐reducing sediment from Aarhus Bay, Denmark. Sediment was incubated in medium containing 10 mM sulfate and different <sup>13</sup>C‐acetate (10, 1, 0.1 mM) concentrations. The resultant changes in microbial community composition were monitored in total and SIP‐fractionated DNA during long‐term incubations. Chemical analyses demonstrated metabolic activity in all sediment slurries, with sulfate‐reducing activity largely determined by initial acetate concentrations. Sequencing of 16S rRNA gene PCR amplicons showed that the incubations shifted the bacterial but not the archaeal community composition. After 3 months of incubation, only sediment slurries incubated with 10 mM <sup>13</sup>C‐acetate showed detectable <sup>13</sup>C‐DNA labeling. Based on 16S rRNA and <italic>dsr</italic><italic>B</italic> gene PCR amplicon sequencing, the <sup>13</sup>C‐labeled DNA pool was dominated by a single type of sulfate reducer representing a novel genus in the family <italic>D</italic><italic>esulfobacteraceae.</italic> In addition, members of the uncultivated Crenarchaeotal group C3 were enriched in the <sup>13</sup>C‐labeled DNA. Our results were reproducible across biological replicate experiments and provide new information about the identities of uncultured acetate‐consuming bacteria and archaea in marine sediments.</p> </abstract>