Effect of exogenous xylanase on rumen in vitro gas production and degradability of wheat straw. (29th April 2015)

• Record Type: Journal Article
• Authors: Togtokhbayar, Norovsambuu; Cerrillo, María A.; Rodríguez, Germán Buendía; Elghandour, Mona M. M. Y.; Salem, Abdelfattah Z. M.; Urankhaich, Chuluunbaatar; Jigjidpurev, Sukhbaatar; Odongo, Nicholas E.; Kholif, Ahmed E.
• Is Part Of: Animal science journal. Volume 86:Number 8(2015:Aug.)
• Page Start: 765
• Page End: 771
• Publication Date: 2015-04-29
• Subjects: Animal culture -- Periodicals; Livestock -- Periodicals; 636
• DOI: 10.1111/asj.12364
• Languages: English
• ISSNs: 1344-3941

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>The objective of this study was to determine effects of xylanase on <italic>in vitro</italic> gas production (GP) and <italic>in sacco</italic> degradability of wheat straw. Rumen fluid was obtained from three Mongolian native goats fitted with permanent rumen cannulas. The trial consisted of five doses (0, 0.5, 1.0, 1.5, 2.0 μL/g of substrate) of a commercial xylanase (Dyadic<sup>®</sup> xylanase PLUS, Dyadic International, Inc., Jupiter, FL, USA). For the <italic>in sacco</italic> degradability, different levels of xylanase enzyme were added directly onto 2 g of wheat straw in nylon bags and incubated in the rumen for 3, 6, 12, 24 and 48 h to estimate degradability of wheat straw. Total GP increased (<italic>P</italic> < 0.001) at all times of incubation at intermediate levels of xylanase. Methane production had a similar pattern at 3 and 12 h of incubation; increased linearly at 24 h of incubation, and was unaffected at 6 and 48 h of incubation. Rumen NH<sub>3</sub>‐N concentration increased linearly at 3 h and the highest values were observed with intermediate enzyme levels. All ruminal volatile fatty acids increased linearly with intermediate levels of the fibrolytic enzyme. The <italic>in sacco</italic> rate of dry matter degradation decreased linearly (<italic>P</italic> = 0.020) with increasing enzymes. Intermediate levels of xylanase improved rumen kinetic fermentation and degradability. The outcome of this research indicated that the application of xylanase enzyme could improve <italic>in vitro</italic> GP fermentation of wheat straw.</p> </abstract>